Detailed information of TA system TA123820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 255361..256022
• Replicon: chromosome
• Accession: NZ_CP039339
• Organism: Ralstonia solanacearum strain UW386

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: E7Z57_RS01370
• Protein ID: WP_138928271.1
• Coordinates: 255361..255774 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D8N5P4
• Locus tag: E7Z57_RS01375
• Protein ID: WP_020749302.1
• Coordinates: 255771..256022 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7Z57_RS01335	250873..251643	+	771	WP_138928266.1	hypothetical protein	-
E7Z57_RS01340	251652..252044	+	393	WP_138928267.1	hypothetical protein	-
E7Z57_RS01345	252041..252262	+	222	WP_138928268.1	hypothetical protein	-
E7Z57_RS01350	252237..252881	+	645	WP_138928269.1	hypothetical protein	-
E7Z57_RS01355	252924..253214	-	291	WP_064051869.1	H-NS histone family protein	-
E7Z57_RS01360	253409..254881	-	1473	WP_138928270.1	YOPP/AvrRxv family protein	-
E7Z57_RS01365	255130..255356	+	227	Protein_260	hypothetical protein	-
E7Z57_RS01370	255361..255774	-	414	WP_138928271.1	type II toxin-antitoxin system VapC family toxin	Toxin
E7Z57_RS01375	255771..256022	-	252	WP_020749302.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E7Z57_RS01380	256098..256721	+	624	Protein_263	tape measure protein	-
E7Z57_RS24670	256707..257003	+	297	Protein_264	lysozyme	-
E7Z57_RS01385	257071..257367	+	297	WP_138928273.1	hypothetical protein	-
E7Z57_RS01390	257421..257759	-	339	WP_138928274.1	DUF1484 domain-containing protein	-
E7Z57_RS01395	258221..258556	+	336	WP_138928275.1	helix-turn-helix domain-containing protein	-
E7Z57_RS01400	258571..259713	-	1143	WP_138928276.1	ABC transporter substrate-binding protein	-
E7Z57_RS01405	259969..260361	-	393	WP_138928277.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	234138..262928	28790

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-63)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15784.17 Da
• Isoelectric Point: 6.2337

>T123820 WP_138928271.1 NZ_CP039339:c255774-255361 [Ralstonia solanacearum]
MSYLIDTNVLSELRRKQPDARVLAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAVRRQHLIDWLETELPNYFLGRLLDID
AHTADRWGRLMSSANRPLPAIDGLLAATALQHDLILVTRNTKDFVGLDVPLINPWET

Nucleotide

• Length: 414 bp

>T123820 NZ_CP039339:c255774-255361 [Ralstonia solanacearum]
TTGAGCTACCTGATCGACACCAATGTGCTGTCCGAGCTACGCCGCAAGCAGCCCGATGCGCGCGTGCTCGCCTGGATGCA
AGACCGGCCGCGCCAGTCGCTCTACCTGAGCGTGCTGACCCTGGGCGAGATCCGCAAAGGCATTGAGCGGCTGGATGACG
CGGTACGCCGCCAGCACCTGATCGACTGGCTCGAAACAGAATTGCCGAACTACTTCCTCGGCCGCTTGCTCGACATCGAT
GCCCATACCGCCGACCGCTGGGGCCGGCTGATGTCGAGCGCCAACCGGCCGCTGCCGGCGATCGATGGCCTGCTCGCCGC
GACCGCCCTGCAGCACGACCTCATACTGGTCACGCGCAACACGAAGGATTTCGTCGGACTCGATGTCCCGCTCATCAACC
CGTGGGAAACGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9258.46 Da
• Isoelectric Point: 5.0628

>AT123820 WP_020749302.1 NZ_CP039339:c256022-255771 [Ralstonia solanacearum]
MQSWQLQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGSGESLVDFMRQSPLAGLDDVVFERERSLPRE
VDF

Nucleotide

• Length: 252 bp

>AT123820 NZ_CP039339:c256022-255771 [Ralstonia solanacearum]
ATGCAAAGCTGGCAGTTGCAAGCCGCCAAGGCGCGGTTTTCCGACGTCGTGAAGCGGGCAGCGGACGATGGTCCGCAGGA
AATCACGGTGCATGGCCGCCCGGTTGCGGTTGTGATCTCACGCGCACTGTTCGATCGCTTGAGTGGCAGCGGGGAGTCGC
TGGTCGACTTCATGCGCCAGTCTCCGTTGGCTGGCCTGGACGACGTCGTCTTCGAGCGCGAGCGCAGCCTGCCGCGCGAG
GTCGACTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A5B7U0Z6