Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7604..8247 Replicon plasmid unnamed2
Accession NZ_CP039329
Organism Citrobacter portucalensis strain Effluent_1

Toxin (Protein)


Gene name vagD Uniprot ID D7Y5A6
Locus tag E7703_RS25320 Protein ID WP_001044765.1
Coordinates 7831..8247 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag E7703_RS25315 Protein ID WP_001261282.1
Coordinates 7604..7834 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E7703_RS25285 3117..3383 - 267 WP_024144810.1 type II toxin-antitoxin system ParD family antitoxin -
E7703_RS25290 3614..5287 - 1674 WP_000026497.1 hypothetical protein -
E7703_RS25300 5852..6661 - 810 WP_000654269.1 hypothetical protein -
E7703_RS25305 6679..7041 - 363 WP_000533745.1 hypothetical protein -
E7703_RS25310 7225..7647 - 423 WP_162534719.1 hypothetical protein -
E7703_RS25315 7604..7834 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E7703_RS25320 7831..8247 + 417 WP_001044765.1 type II toxin-antitoxin system VapC family toxin Toxin
E7703_RS25325 8321..9883 + 1563 WP_001128475.1 AAA family ATPase -
E7703_RS25330 9868..10890 + 1023 WP_000361404.1 helicase UvrD -
E7703_RS25345 11819..12040 + 222 Protein_12 IS5/IS1182 family transposase -
E7703_RS25355 12189..12506 - 318 Protein_13 hypothetical protein -
E7703_RS25360 12503..12988 - 486 WP_000045622.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..66363 66363


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15148.60 Da        Isoelectric Point: 7.1082

>T123818 WP_001044765.1 NZ_CP039329:7831-8247 [Citrobacter portucalensis]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T123818 NZ_CP039329:7831-8247 [Citrobacter portucalensis]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT123818 WP_001261282.1 NZ_CP039329:7604-7834 [Citrobacter portucalensis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT123818 NZ_CP039329:7604-7834 [Citrobacter portucalensis]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9AY90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References