Detailed information of TA system TA123817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 122366..122891
• Replicon: plasmid unnamed1
• Accession: NZ_CP039328
• Organism: Citrobacter portucalensis strain Effluent_1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: E7703_RS25245
• Protein ID: WP_016946795.1
• Coordinates: 122586..122891 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: E7703_RS25240
• Protein ID: WP_023294775.1
• Coordinates: 122366..122584 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7703_RS25225	117635..118498	-	864	WP_136392547.1	DUF4231 domain-containing protein	-
E7703_RS25230	118515..119072	-	558	WP_042005136.1	recombinase family protein	-
E7703_RS25235	119233..122244	+	3012	WP_136392548.1	Tn3 family transposase	-
E7703_RS25240	122366..122584	+	219	WP_023294775.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
E7703_RS25245	122586..122891	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
E7703_RS25250	123049..123555	+	507	WP_023294776.1	hypothetical protein	-
E7703_RS25255	123734..124510	+	777	WP_049245386.1	site-specific integrase	-
E7703_RS25260	124568..124825	-	258	WP_000764642.1	hypothetical protein	-
E7703_RS25895	124954..125058	-	105	WP_032409716.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..125137	125137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T123817 WP_016946795.1 NZ_CP039328:122586-122891 [Citrobacter portucalensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T123817 NZ_CP039328:122586-122891 [Citrobacter portucalensis]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGTCGCTATCGTCTGTTCGTGGATGTACAGAGTGACATCATCGATAC
CCCCGGGCGGCGCATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTTTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7558

>AT123817 WP_023294775.1 NZ_CP039328:122366-122584 [Citrobacter portucalensis]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFAEENRDW

Nucleotide

• Length: 219 bp

>AT123817 NZ_CP039328:122366-122584 [Citrobacter portucalensis]
ATGAAGCAGCGCATCACCGTCACAATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATATCCGGTCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGCCGTTTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGGTTTATTGAAATGAACGGTTCATTCGCCGAAGAAAACAGGGACTGGTGA