Detailed information of TA system TA123812

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3432546..3433210
• Replicon: chromosome
• Accession: NZ_CP039327
• Organism: Citrobacter portucalensis strain Effluent_1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: E7703_RS17005
• Protein ID: WP_136392185.1
• Coordinates: 3432884..3433210 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: E7703_RS17000
• Protein ID: WP_136392184.1
• Coordinates: 3432546..3432863 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7703_RS16955	3427984..3428805	+	822	WP_008324532.1	DUF945 domain-containing protein	-
E7703_RS16960	3429023..3429724	+	702	WP_104652023.1	WYL domain-containing protein	-
E7703_RS16965	3429761..3429997	+	237	WP_063255810.1	hypothetical protein	-
E7703_RS16970	3429997..3430440	+	444	WP_136392181.1	hypothetical protein	-
E7703_RS16975	3430464..3430931	+	468	WP_136392182.1	hypothetical protein	-
E7703_RS16980	3431008..3431247	+	240	WP_063199992.1	DUF905 domain-containing protein	-
E7703_RS16985	3431345..3431803	+	459	WP_000211838.1	antirestriction protein	-
E7703_RS16990	3431819..3432295	+	477	WP_046495838.1	RadC family protein	-
E7703_RS16995	3432304..3432525	+	222	WP_136392183.1	DUF987 domain-containing protein	-
E7703_RS17000	3432546..3432863	+	318	WP_136392184.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E7703_RS17005	3432884..3433210	+	327	WP_136392185.1	TA system toxin CbtA family protein	Toxin
E7703_RS17010	3433452..3434084	-	633	WP_048215836.1	DNA-binding protein	-
E7703_RS17015	3434271..3436169	-	1899	WP_136392186.1	methyl-accepting chemotaxis protein	-
E7703_RS17020	3436540..3437079	+	540	WP_020996692.1	DUF2058 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3424986..3433210	8224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-106)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12321.25 Da
• Isoelectric Point: 8.0567

>T123812 WP_136392185.1 NZ_CP039327:3432884-3433210 [Citrobacter portucalensis]
MKTLAATISRATKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYIRAVDILRARQTLGLLRGSH

Nucleotide

• Length: 327 bp

>T123812 NZ_CP039327:3432884-3433210 [Citrobacter portucalensis]
ATGAAAACTCTAGCTGCAACAATTTCGCGGGCGACGAAGCCCTGTCTGTCGCCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCATTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGTATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
AGCTGGCAAGAACAATCCCCTTATATTCGGGCTGTTGATATTTTGCGAGCTCGGCAGACACTAGGTCTTTTACGAGGATC
TCATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12159.81 Da
• Isoelectric Point: 7.8418

>AT123812 WP_136392184.1 NZ_CP039327:3432546-3432863 [Citrobacter portucalensis]
MTHNTWGLQRNITPRLGVRLVQERNRLHYLADRASITGKFSDAEYRKLDETFPHFIRQMESMLTTAELSPQHAHCVILYH
NGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT123812 NZ_CP039327:3432546-3432863 [Citrobacter portucalensis]
ATGACTCACAACACATGGGGCCTGCAGCGGAATATCACGCCGCGCCTGGGAGTCCGTCTGGTGCAGGAGCGGAACCGGCT
GCATTATCTGGCTGACCGGGCCAGTATTACCGGTAAATTCAGTGACGCCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGCTGAACTCAGCCCCCAACATGCCCACTGCGTCATCCTGTATCAC
AACGGTTTCACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTATGTATATATCGCAGTCTATCCCACTCAACGCTAA