Detailed information of TA system TA123802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2406997..2407733
• Replicon: chromosome
• Accession: NZ_CP039327
• Organism: Citrobacter portucalensis strain Effluent_1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5B0TAB3
• Locus tag: E7703_RS11910
• Protein ID: WP_048214201.1
• Coordinates: 2406997..2407479 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: E7703_RS11915
• Protein ID: WP_008784769.1
• Coordinates: 2407467..2407733 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7703_RS11880	2402564..2403085	+	522	WP_048226507.1	GNAT family N-acetyltransferase	-
E7703_RS11885	2403095..2403931	-	837	WP_136391870.1	DMT family transporter	-
E7703_RS11895	2404160..2405128	+	969	WP_072202995.1	IS5 family transposase	-
E7703_RS11900	2405184..2405426	-	243	Protein_2296	hypothetical protein	-
E7703_RS11905	2405807..2406649	+	843	Protein_2297	mechanosensitive ion channel protein MscS	-
E7703_RS11910	2406997..2407479	-	483	WP_048214201.1	GNAT family N-acetyltransferase	Toxin
E7703_RS11915	2407467..2407733	-	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
E7703_RS11920	2407887..2409869	-	1983	WP_071692396.1	MBL fold metallo-hydrolase	-
E7703_RS11925	2410113..2410499	+	387	WP_136391872.1	hypothetical protein	-
E7703_RS11930	2410616..2411245	-	630	WP_136391873.1	winged helix-turn-helix transcriptional regulator	-
E7703_RS11935	2411485..2412672	-	1188	WP_136391874.1	outer membrane protein transport protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2403095..2411245	8150
	-	flank	IS/Tn	-	-	2404205..2405128	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17572.35 Da
• Isoelectric Point: 9.1059

>T123802 WP_048214201.1 NZ_CP039327:c2407479-2406997 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGATRTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHVLTDEAKRFYLHHGFKASQLQERTLFLGLS

Nucleotide

• Length: 483 bp

>T123802 NZ_CP039327:c2407479-2406997 [Citrobacter portucalensis]
GTGGGATTTGTAACCGCACCTGAGCCACTAACTCACATTCATCAATTGGCGGAATTTGTCAGCGGAGAAATTGTTCTAGA
TGATTGGTTAAAGCAAAAAGGGTTGAAAAATCAGGCTCTCGGTGCAACCAGGACGTTTGTTGTCTGCAAAACAGACACGC
GACAAGTCGCTGGCTTTTATTCACTGGCCACTGGTAGCGTCAACCACACAGAAGCAACAGGCAATCTTCGGCGTAACATG
CCAGATCCAATCCCCGTAATTATCCTTGCCAGATTGGCCGTAGATCTTTCTGTTCGTGGAAAAGGGTTAGGGGCCGATCT
GCTTCACGATGCGGTACTGCGGTGTTACAAAGTGGCAGAAAATATTGGCGTGCGTGCCGTTATGGTGCATGTGCTCACAG
ACGAAGCTAAACGTTTTTATCTTCACCACGGATTCAAAGCATCTCAACTGCAGGAGCGAACTCTGTTCCTTGGGCTTTCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT123802 WP_008784769.1 NZ_CP039327:c2407733-2407467 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT123802 NZ_CP039327:c2407733-2407467 [Citrobacter portucalensis]
ATGAAATCAGATGTCCAGCTCAACCTTAGAGCTAAAGAATCCCAACGCGCGCTTATTGACGCGGCCGCAGAGATTCTACA
TAAATCGCGTACAGACTTTATTCTGGAAATGGCCTGCCAGGCGGCAGAGAACGTCATTCTCGATCGCCGAGTGTTTAATT
TTAATGATGAACAATACGCTGAGTTTATCGAGATGCTTGATGCTCCGGTTGCCGAAGATCCCGCTATCAGGAAGCTACTG
GCAAGGAAACCACAGTGGGATTTGTAA

Structures

Toxin

• AlphaFold DB: A0A5B0TAB3