Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 60026..60552 Replicon plasmid unnamed1
Accession NZ_CP039299
Organism Escherichia coli strain PigCaeca_1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag E7Z58_RS23790 Protein ID WP_000323025.1
Coordinates 60265..60552 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag E7Z58_RS23785 Protein ID WP_000534858.1
Coordinates 60026..60265 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E7Z58_RS23765 55808..57016 - 1209 WP_001493816.1 IncI1-type conjugal transfer protein TrbA -
- 57196..57256 - 61 NuclAT_0 - -
- 57196..57256 - 61 NuclAT_0 - -
- 57196..57256 - 61 NuclAT_0 - -
- 57196..57256 - 61 NuclAT_0 - -
E7Z58_RS23770 57384..58724 + 1341 WP_000589013.1 ISNCY family transposase -
E7Z58_RS23775 59161..59493 - 333 WP_001301033.1 protein FlxA -
E7Z58_RS23780 59696..60001 - 306 WP_071593935.1 hypothetical protein -
E7Z58_RS23785 60026..60265 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
E7Z58_RS23790 60265..60552 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
E7Z58_RS23795 60624..60776 + 153 WP_001387489.1 Hok/Gef family protein -
E7Z58_RS23800 60848..61099 - 252 WP_024171163.1 hypothetical protein -
- 61493..61544 - 52 NuclAT_1 - -
- 61493..61544 - 52 NuclAT_1 - -
- 61493..61544 - 52 NuclAT_1 - -
- 61493..61544 - 52 NuclAT_1 - -
E7Z58_RS24475 62030..62206 - 177 WP_001054900.1 hypothetical protein -
E7Z58_RS23810 62415..62624 - 210 WP_001140543.1 hemolysin expression modulator Hha -
E7Z58_RS23815 62722..63384 - 663 WP_060527638.1 plasmid IncI1-type surface exclusion protein ExcA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 - 1..105771 105771


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T123799 WP_000323025.1 NZ_CP039299:60265-60552 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T123799 NZ_CP039299:60265-60552 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT123799 WP_000534858.1 NZ_CP039299:60026-60265 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT123799 NZ_CP039299:60026-60265 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References