Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 723266..723904 Replicon chromosome
Accession NZ_CP039298
Organism Escherichia coli strain PigCaeca_1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag E7Z58_RS03705 Protein ID WP_000813794.1
Coordinates 723728..723904 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag E7Z58_RS03700 Protein ID WP_001270286.1
Coordinates 723266..723682 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E7Z58_RS03680 718418..719359 - 942 WP_001251313.1 ABC transporter permease -
E7Z58_RS03685 719360..720373 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
E7Z58_RS03690 720391..721536 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
E7Z58_RS03695 721781..723187 - 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
E7Z58_RS03700 723266..723682 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
E7Z58_RS03705 723728..723904 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
E7Z58_RS03710 724126..724356 + 231 WP_000494244.1 YncJ family protein -
E7Z58_RS03715 724448..726409 - 1962 WP_094303868.1 23S rRNA 5-hydroxycytidine C2501 synthase -
E7Z58_RS03720 726482..727018 - 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
E7Z58_RS03725 727071..728285 + 1215 WP_071597387.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 728325..729590 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T123789 WP_000813794.1 NZ_CP039298:c723904-723728 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T123789 NZ_CP039298:c723904-723728 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT123789 WP_001270286.1 NZ_CP039298:c723682-723266 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT123789 NZ_CP039298:c723682-723266 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References