Detailed information of TA system TA123777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 490832..491469
• Replicon: chromosome
• Accession: NZ_CP039297
• Organism: Bacillus velezensis isolate UFLA258

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: E4T61_RS02475
• Protein ID: WP_003156187.1
• Coordinates: 491119..491469 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: E4T61_RS02470
• Protein ID: WP_003156188.1
• Coordinates: 490832..491113 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4T61_RS02450	487197..487796	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
E4T61_RS02455	487889..488254	+	366	WP_007410229.1	holo-ACP synthase	-
E4T61_RS02460	488419..489426	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
E4T61_RS02465	489543..490712	+	1170	WP_025284272.1	alanine racemase	-
E4T61_RS02470	490832..491113	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
E4T61_RS02475	491119..491469	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
E4T61_RS02480	491587..492408	+	822	WP_003156182.1	STAS domain-containing protein	-
E4T61_RS02485	492413..492778	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
E4T61_RS02490	492781..493182	+	402	WP_003156178.1	anti-sigma regulatory factor	-
E4T61_RS02495	493194..494201	+	1008	WP_015239197.1	PP2C family protein-serine/threonine phosphatase	-
E4T61_RS02500	494265..494594	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
E4T61_RS02505	494591..495073	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
E4T61_RS02510	495039..495827	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
E4T61_RS02515	495827..496429	+	603	WP_136396117.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T123777 WP_003156187.1 NZ_CP039297:491119-491469 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T123777 NZ_CP039297:491119-491469 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT123777 WP_003156188.1 NZ_CP039297:490832-491113 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT123777 NZ_CP039297:490832-491113 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5