Detailed information of TA system TA123759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3086847..3087466
• Replicon: chromosome
• Accession: NZ_CP039296
• Organism: Enterococcus faecalis strain VE14089

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: E7T02_RS16355
• Protein ID: WP_000241511.1
• Coordinates: 3086847..3087209 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3H5D1
• Locus tag: E7T02_RS16360
• Protein ID: WP_000245205.1
• Coordinates: 3087203..3087466 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7T02_RS16340	3082257..3084188	-	1932	WP_002370258.1	sucrose-specific PTS transporter subunit IIBC	-
E7T02_RS16345	3084379..3085818	+	1440	WP_002367771.1	sucrose-6-phosphate hydrolase	-
E7T02_RS16350	3085820..3086782	+	963	WP_002367770.1	LacI family DNA-binding transcriptional regulator	-
E7T02_RS16355	3086847..3087209	-	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E7T02_RS16360	3087203..3087466	-	264	WP_000245205.1	PbsX family transcriptional regulator	Antitoxin
E7T02_RS16365	3087643..3088263	+	621	WP_021732893.1	recombinase family protein	-
E7T02_RS16370	3088280..3088567	+	288	WP_010708491.1	hypothetical protein	-
E7T02_RS16375	3088561..3088785	+	225	WP_010708492.1	hypothetical protein	-
E7T02_RS16380	3088846..3089055	+	210	WP_002387638.1	hypothetical protein	-
E7T02_RS16385	3089067..3089369	+	303	WP_002365939.1	hypothetical protein	-
E7T02_RS16390	3089796..3091124	+	1329	WP_002387639.1	Y-family DNA polymerase	-
E7T02_RS16395	3091121..3091471	+	351	WP_002365943.1	hypothetical protein	-
E7T02_RS16405	3091722..3091832	+	111	WP_107164701.1	type I toxin-antitoxin system Fst family toxin	-
-	3091872..3091974	-	103	NuclAT_2	-	-
-	3091872..3091974	-	103	NuclAT_2	-	-
-	3091872..3091974	-	103	NuclAT_2	-	-
-	3091872..3091974	-	103	NuclAT_2	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T123759 WP_000241511.1 NZ_CP039296:c3087209-3086847 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T123759 NZ_CP039296:c3087209-3086847 [Enterococcus faecalis]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10059.46 Da
• Isoelectric Point: 5.1585

>AT123759 WP_000245205.1 NZ_CP039296:c3087466-3087203 [Enterococcus faecalis]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Nucleotide

• Length: 264 bp

>AT123759 NZ_CP039296:c3087466-3087203 [Enterococcus faecalis]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTGCATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Structures

Toxin

• AlphaFold DB: R3H4V2

Antitoxin

• AlphaFold DB: R3H5D1