Detailed information of TA system TA123745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5606409..5606995
• Replicon: chromosome
• Accession: NZ_CP039293
• Organism: Pseudomonas aeruginosa strain PABL048

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: DZ927_RS26975
• Protein ID: WP_003120987.1
• Coordinates: 5606696..5606995 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DZ927_RS26970
• Protein ID: WP_003448662.1
• Coordinates: 5606409..5606699 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZ927_RS26950	5601560..5601769	+	210	WP_003105733.1	cold-shock protein	-
DZ927_RS26955	5601991..5603880	+	1890	WP_016851610.1	hypothetical protein	-
DZ927_RS26960	5603877..5605853	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
DZ927_RS26965	5605994..5606338	+	345	WP_016851612.1	hypothetical protein	-
DZ927_RS26970	5606409..5606699	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
DZ927_RS26975	5606696..5606995	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DZ927_RS26980	5607197..5608321	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
DZ927_RS26985	5608321..5610030	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
DZ927_RS26990	5610034..5611359	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
DZ927_RS26995	5611349..5611882	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5578776..5666623	87847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T123745 WP_003120987.1 NZ_CP039293:c5606995-5606696 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T123745 NZ_CP039293:c5606995-5606696 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT123745 WP_003448662.1 NZ_CP039293:c5606699-5606409 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT123745 NZ_CP039293:c5606699-5606409 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73