Detailed information of TA system TA123743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 5154886..5155459
• Replicon: chromosome
• Accession: NZ_CP039293
• Organism: Pseudomonas aeruginosa strain PABL048

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W5IUR0
• Locus tag: DZ927_RS24845
• Protein ID: WP_009618209.1
• Coordinates: 5154886..5155173 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W5ISI7
• Locus tag: DZ927_RS24850
• Protein ID: WP_009618210.1
• Coordinates: 5155160..5155459 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZ927_RS24820	5150528..5152543	-	2016	WP_013982117.1	DNA topoisomerase III	-
DZ927_RS24825	5152838..5153221	-	384	WP_004362284.1	single-stranded DNA-binding protein	-
DZ927_RS24830	5153218..5153766	-	549	WP_031641644.1	DUF3158 family protein	-
DZ927_RS24835	5153763..5154542	-	780	WP_013982115.1	TIGR03761 family integrating conjugative element protein	-
DZ927_RS24840	5154666..5154857	-	192	WP_011805449.1	hypothetical protein	-
DZ927_RS24845	5154886..5155173	-	288	WP_009618209.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DZ927_RS24850	5155160..5155459	-	300	WP_009618210.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DZ927_RS24855	5155587..5156780	-	1194	WP_013982114.1	hypothetical protein	-
DZ927_RS24860	5156783..5157343	-	561	WP_013982113.1	DUF2857 domain-containing protein	-
DZ927_RS24865	5157361..5159019	-	1659	WP_013982112.1	ParB family protein	-
DZ927_RS24870	5159012..5159248	-	237	WP_013982111.1	type II toxin-antitoxin system HicA family toxin	-
DZ927_RS24875	5159232..5160098	-	867	WP_013982110.1	ParA family protein	-
DZ927_RS24880	5160139..5160357	-	219	WP_023084999.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5080588..5222560	141972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-58)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10854.46 Da
• Isoelectric Point: 8.4628

>T123743 WP_009618209.1 NZ_CP039293:c5155173-5154886 [Pseudomonas aeruginosa]
VRVLEWREAARADLLAIVDYISDDNPDAAQRLKDDIEAKAAKLPERPKLYRPGRVAGTREMVVRANYVVVYMEDTRAVSI
LRVLHAAQQWPPARE

Nucleotide

• Length: 288 bp

>T123743 NZ_CP039293:c5155173-5154886 [Pseudomonas aeruginosa]
GTGCGCGTACTTGAGTGGCGGGAAGCTGCCCGCGCCGACCTGCTGGCGATTGTTGACTACATCTCGGACGACAACCCCGA
TGCCGCGCAGCGGTTGAAGGACGACATCGAGGCCAAGGCCGCGAAGCTGCCGGAGCGCCCGAAGCTCTACCGGCCTGGCC
GCGTGGCCGGCACGCGGGAGATGGTCGTGCGTGCGAACTACGTGGTGGTCTACATGGAGGACACCCGCGCGGTGTCCATC
CTGCGCGTGTTGCACGCCGCGCAGCAGTGGCCGCCGGCACGGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10816.46 Da
• Isoelectric Point: 8.6579

>AT123743 WP_009618210.1 NZ_CP039293:c5155459-5155160 [Pseudomonas aeruginosa]
MAAQTSMLHIRVDDKLKADAAEKLAGVGLTVSDAVRILLTRVVKEGALPAGLTADPEAYDAWFRAKVQEALADTRPPVPH
QQVMDEAQALIDRKRRART

Nucleotide

• Length: 300 bp

>AT123743 NZ_CP039293:c5155459-5155160 [Pseudomonas aeruginosa]
ATGGCCGCACAGACTTCGATGCTGCACATCCGCGTGGACGATAAGCTCAAGGCCGATGCAGCCGAGAAACTCGCCGGGGT
CGGCCTCACCGTTTCGGACGCCGTGCGCATCCTGCTGACCCGTGTGGTCAAGGAAGGCGCACTGCCGGCTGGCCTCACGG
CCGACCCGGAGGCCTACGATGCCTGGTTCCGGGCGAAGGTCCAGGAGGCGCTGGCCGACACACGGCCGCCGGTGCCGCAC
CAGCAGGTCATGGATGAGGCGCAGGCCCTGATCGACAGGAAGCGGCGTGCGCGTACTTGA

Structures

Toxin

• AlphaFold DB: A0A5R9QF16

Antitoxin

• AlphaFold DB: A0A5R9QE11