Detailed information of TA system TA123739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2751617..2752659
• Replicon: chromosome
• Accession: NZ_CP039293
• Organism: Pseudomonas aeruginosa strain PABL048

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: DZ927_RS13565
• Protein ID: WP_003153636.1
• Coordinates: 2752084..2752659 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: DZ927_RS13560
• Protein ID: WP_003050245.1
• Coordinates: 2751617..2752087 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZ927_RS13525	2747009..2748427	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
DZ927_RS13530	2748417..2749328	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
DZ927_RS13535	2749325..2750017	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
DZ927_RS13540	2750014..2750412	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
DZ927_RS13545	2750424..2750783	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
DZ927_RS13550	2750800..2751033	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
DZ927_RS13555	2751030..2751413	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
DZ927_RS13560	2751617..2752087	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
DZ927_RS13565	2752084..2752659	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
DZ927_RS13570	2752677..2753591	+	915	WP_016852809.1	AAA family ATPase	-
DZ927_RS13575	2753588..2754058	+	471	WP_003090160.1	hypothetical protein	-
DZ927_RS13580	2754055..2754555	+	501	WP_003090159.1	hypothetical protein	-
DZ927_RS13585	2754555..2755457	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
DZ927_RS13590	2755496..2756221	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2677052..2801518	124466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T123739 WP_003153636.1 NZ_CP039293:2752084-2752659 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T123739 NZ_CP039293:2752084-2752659 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT123739 WP_003050245.1 NZ_CP039293:2751617-2752087 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT123739 NZ_CP039293:2751617-2752087 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68