123730

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2686863..2687506 Replicon chromosome
Accession NZ_CP039288
Organism Cupriavidus necator H16

Toxin (Protein)


Gene name hicA Uniprot ID A0A7T4KLX9
Locus tag E6A55_RS31185 Protein ID WP_041688562.1
Coordinates 2686863..2687039 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R7XL07
Locus tag E6A55_RS31190 Protein ID WP_010810694.1
Coordinates 2687090..2687506 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6A55_RS31165 2682497..2683315 + 819 WP_011617819.1 flagellar assembly protein FliH -
E6A55_RS31170 2683308..2684783 + 1476 WP_010810690.1 flagellar protein export ATPase FliI -
E6A55_RS31175 2684761..2685219 + 459 WP_011617820.1 flagellar export protein FliJ -
E6A55_RS31180 2685245..2686663 + 1419 WP_011617821.1 flagellar hook-length control protein FliK -
E6A55_RS31185 2686863..2687039 + 177 WP_041688562.1 type II toxin-antitoxin system HicA family toxin Toxin
E6A55_RS31190 2687090..2687506 + 417 WP_010810694.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E6A55_RS31195 2687537..2688199 + 663 WP_011617823.1 DNA oxidative demethylase AlkB -
E6A55_RS31200 2688284..2688772 + 489 WP_041688226.1 GFA family protein -
E6A55_RS31205 2688822..2689739 - 918 WP_010810697.1 cysteine synthase A -
E6A55_RS31210 2690120..2691112 + 993 WP_051398583.1 site-specific integrase -
E6A55_RS31220 2691437..2692171 + 735 WP_041688227.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(87-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6638.70 Da        Isoelectric Point: 12.0931

>T123730 WP_041688562.1 NZ_CP039288:2686863-2687039 [Cupriavidus necator H16]
VKRSEFRRWLAGQGATFSEGAKHVRVYLNGRKTTLPRHPSHEIREGLRKAILKQLGLS

Download         Length: 177 bp

>T123730 NZ_CP039288:2686863-2687039 [Cupriavidus necator H16]
GTGAAGCGGAGTGAATTCCGGCGATGGCTCGCCGGACAGGGCGCCACCTTCAGTGAAGGCGCGAAGCATGTGAGGGTGTA
TCTCAATGGCAGGAAGACCACGCTGCCACGGCATCCCAGCCACGAGATCCGGGAGGGGTTGCGCAAGGCGATTCTCAAGC
AACTCGGCCTGTCATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15083.34 Da        Isoelectric Point: 5.2420

>AT123730 WP_010810694.1 NZ_CP039288:2687090-2687506 [Cupriavidus necator H16]
MLRFPANIVPDDGGFAVSFPDIPEALTSGDTIEQAREMAADALATAMEFYFEDGRPVPLPSKAKRGQHLVELPASVSAKV
LLLNEMIAQGVTQAELARRLNTRKQEVQRIVNLDHATKIDTIEAAFRALGKRLELTVA

Download         Length: 417 bp

>AT123730 NZ_CP039288:2687090-2687506 [Cupriavidus necator H16]
ATGCTGCGTTTCCCTGCCAACATCGTCCCGGACGACGGTGGCTTTGCTGTGTCGTTTCCCGATATCCCCGAAGCCCTGAC
CAGCGGCGACACCATCGAGCAGGCCCGCGAAATGGCCGCCGATGCGCTCGCCACGGCCATGGAATTCTATTTCGAAGATG
GCCGCCCGGTGCCACTGCCGTCGAAGGCAAAACGCGGCCAGCATCTGGTTGAACTGCCGGCCAGCGTGTCGGCGAAGGTG
CTCCTGCTTAACGAGATGATCGCGCAGGGCGTGACGCAGGCGGAACTGGCGCGCCGGCTGAATACGCGCAAGCAAGAGGT
GCAACGTATCGTCAACCTGGACCATGCGACCAAGATCGATACGATCGAGGCTGCATTCCGGGCGCTGGGGAAGCGGCTGG
AACTGACGGTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T4KLX9

Antitoxin

Source ID Structure
AlphaFold DB A0A7T4KMX9

References