Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2264690..2265348 Replicon chromosome
Accession NZ_CP039288
Organism Cupriavidus necator H16

Toxin (Protein)


Gene name vapC Uniprot ID R7XG42
Locus tag E6A55_RS29360 Protein ID WP_010812752.1
Coordinates 2264690..2265100 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R7XF63
Locus tag E6A55_RS29365 Protein ID WP_010812751.1
Coordinates 2265097..2265348 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6A55_RS29340 2260307..2261281 - 975 WP_010812756.1 LysR family transcriptional regulator -
E6A55_RS29345 2261372..2262601 + 1230 WP_011617587.1 CoA transferase -
E6A55_RS29350 2262598..2263608 + 1011 WP_010812754.1 NADPH:quinone oxidoreductase family protein -
E6A55_RS29355 2263662..2264609 + 948 WP_051398610.1 tripartite tricarboxylate transporter substrate binding protein -
E6A55_RS29360 2264690..2265100 - 411 WP_010812752.1 type II toxin-antitoxin system VapC family toxin Toxin
E6A55_RS29365 2265097..2265348 - 252 WP_010812751.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E6A55_RS29370 2265766..2266941 + 1176 WP_011617589.1 branched-chain amino acid ABC transporter substrate-binding protein -
E6A55_RS29375 2267022..2267951 + 930 WP_013953596.1 branched-chain amino acid ABC transporter permease -
E6A55_RS29380 2268019..2269149 + 1131 WP_037023464.1 branched-chain amino acid ABC transporter permease -
E6A55_RS29385 2269146..2269928 + 783 WP_011617591.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(8-55)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14457.92 Da        Isoelectric Point: 8.0357

>T123729 WP_010812752.1 NZ_CP039288:c2265100-2264690 [Cupriavidus necator H16]
MTRYMLDTNIVSLLLRAHPAVVARVTAAPMVALCLSAITGAELMYGLAKRPGAARLARAVDELLRRVEVLPWTPSVMAGY
GETRAALESQGQPVGALNLLIAAHALETGAVLVTNDQAFRRVPGLVVEDWTVPTGN

Download         Length: 411 bp

>T123729 NZ_CP039288:c2265100-2264690 [Cupriavidus necator H16]
ATGACGCGCTACATGCTCGACACCAATATCGTGAGCCTTCTGTTGCGGGCACATCCGGCAGTCGTGGCACGCGTGACGGC
AGCGCCGATGGTGGCGCTGTGCCTGTCAGCCATCACCGGTGCGGAACTGATGTACGGGCTGGCCAAGCGTCCCGGCGCAG
CGCGCCTCGCGCGCGCGGTCGACGAGTTGCTGCGCAGGGTCGAGGTATTGCCCTGGACCCCTTCAGTGATGGCCGGCTAT
GGTGAAACCCGCGCTGCGCTGGAATCCCAAGGACAGCCGGTGGGGGCACTGAACCTGCTGATCGCCGCGCACGCGCTGGA
GACTGGCGCGGTGCTGGTGACCAATGACCAGGCATTCCGGCGCGTCCCTGGCCTGGTGGTGGAGGACTGGACGGTTCCGA
CCGGAAATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9298.38 Da        Isoelectric Point: 4.8011

>AT123729 WP_010812751.1 NZ_CP039288:c2265348-2265097 [Cupriavidus necator H16]
VSTTAKIFTTGRSQAVRLPQEFRFAEREVYIRRDPSTGDVILSRRPESWDGFLAAIKDSAVPEDFMADRAQGEQARDPLA
GLE

Download         Length: 252 bp

>AT123729 NZ_CP039288:c2265348-2265097 [Cupriavidus necator H16]
GTGAGCACCACTGCCAAGATCTTCACCACCGGCCGCAGCCAGGCGGTACGGCTGCCGCAGGAATTCCGCTTCGCCGAGCG
CGAGGTATATATTCGCCGTGACCCGTCCACCGGTGACGTGATCCTGTCGCGCCGGCCGGAGAGTTGGGACGGCTTTTTGG
CTGCCATCAAGGACAGCGCCGTGCCGGAAGACTTTATGGCCGACCGCGCGCAGGGCGAGCAAGCCAGGGATCCGCTGGCG
GGTCTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T4FZ50

Antitoxin

Source ID Structure
AlphaFold DB A0A7T4FZ54

References