Detailed information of TA system TA123724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1560898..1561491
• Replicon: chromosome
• Accession: NZ_CP039287
• Organism: Cupriavidus necator H16

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q0KBP4
• Locus tag: E6A55_RS07285
• Protein ID: WP_011615086.1
• Coordinates: 1561192..1561491 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: E6A55_RS07280
• Protein ID: WP_010810129.1
• Coordinates: 1560898..1561188 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6A55_RS07260	1556003..1557772	+	1770	WP_011615085.1	class I poly(R)-hydroxyalkanoic acid synthase	-
E6A55_RS07265	1557857..1559038	+	1182	WP_010810132.1	acetyl-CoA C-acetyltransferase	-
E6A55_RS07270	1559113..1559853	+	741	WP_010810131.1	3-ketoacyl-ACP reductase	-
E6A55_RS07275	1560290..1560841	+	552	WP_010810130.1	polyhydroxyalkanoate synthesis repressor PhaR	-
E6A55_RS07280	1560898..1561188	-	291	WP_010810129.1	putative addiction module antidote protein	Antitoxin
E6A55_RS07285	1561192..1561491	-	300	WP_011615086.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E6A55_RS07290	1561728..1562696	+	969	WP_011615087.1	tRNA-dihydrouridine synthase	-
E6A55_RS07295	1562831..1564210	+	1380	WP_041687282.1	30S ribosomal protein S12 methylthiotransferase RimO	-
E6A55_RS07300	1564302..1565486	+	1185	WP_011615089.1	acetyl-CoA C-acyltransferase family protein	-
E6A55_RS07305	1565495..1566121	+	627	WP_010810124.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11056.61 Da
• Isoelectric Point: 10.1574

>T123724 WP_011615086.1 NZ_CP039287:c1561491-1561192 [Cupriavidus necator H16]
MLSIRTTQISDRWYSALRDHVARIRIQVRVDRLLNGNPGDAKALGGGVSEMRVDHGPGYRIYFQQRGQVIVILLCGGNKS
TQHADIERARSLAANLDLE

Nucleotide

• Length: 300 bp

>T123724 NZ_CP039287:c1561491-1561192 [Cupriavidus necator H16]
ATGCTCAGCATCCGGACAACCCAAATTTCCGACCGCTGGTATTCGGCCTTGCGCGACCACGTCGCCAGGATTCGCATCCA
GGTCCGCGTCGACCGCTTACTGAACGGGAATCCCGGCGACGCTAAAGCCCTTGGCGGTGGAGTCAGCGAAATGCGCGTGG
ACCACGGCCCGGGCTACCGCATCTACTTTCAGCAACGCGGCCAGGTAATTGTGATCCTGCTCTGCGGAGGCAACAAGTCG
ACCCAGCACGCTGATATCGAAAGAGCACGCAGTCTGGCGGCCAATCTTGATCTGGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10607.97 Da
• Isoelectric Point: 6.4635

>AT123724 WP_010810129.1 NZ_CP039287:c1561188-1560898 [Cupriavidus necator H16]
MKEPTRPWDSAEHLRNDQEIAAYLDACLAEAGDDDRFIAYALGVVARARGMTRLARETGLSRESLYRSLSGEGNPEFGTI
WKVMRALGIRLHASAG

Nucleotide

• Length: 291 bp

>AT123724 NZ_CP039287:c1561188-1560898 [Cupriavidus necator H16]
ATGAAGGAACCAACCCGCCCCTGGGACTCCGCCGAGCATCTGAGGAACGATCAGGAGATCGCCGCCTACCTGGATGCCTG
CCTGGCCGAGGCCGGCGACGACGATCGATTCATTGCCTACGCACTCGGGGTGGTGGCGCGTGCGCGCGGCATGACGCGAC
TGGCCCGCGAAACGGGCCTTTCGCGCGAAAGCCTCTACCGCTCGCTCTCAGGCGAAGGCAATCCGGAGTTCGGGACGATC
TGGAAGGTGATGCGGGCGCTGGGCATCCGCCTGCATGCGAGCGCTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A7T4KIZ7