Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazE-MazF
Location 68652..69217 Replicon plasmid pLiSL3
Accession NZ_CP039286
Organism Leptospira interrogans strain FMAS_AW1

Toxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag E4414_RS22590 Protein ID WP_000648189.1
Coordinates 68652..68900 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag E4414_RS22595 Protein ID WP_000237066.1
Coordinates 68894..69217 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4414_RS22560 64269..64763 - 495 WP_017858174.1 hypothetical protein -
E4414_RS22565 65010..65513 + 504 WP_000614121.1 hypothetical protein -
E4414_RS22570 65645..66133 + 489 WP_002131542.1 DUF4942 domain-containing protein -
E4414_RS22575 66253..66498 + 246 WP_000695324.1 hypothetical protein -
E4414_RS22580 67383..67688 + 306 WP_000844596.1 hypothetical protein -
E4414_RS22585 67661..68347 + 687 WP_082278913.1 restriction endonuclease -
E4414_RS22590 68652..68900 + 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Toxin
E4414_RS22595 68894..69217 + 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Antitoxin
E4414_RS22600 69340..69510 + 171 WP_002106501.1 hypothetical protein -
E4414_RS23125 69574..69753 - 180 WP_000536132.1 hypothetical protein -
E4414_RS22605 69669..70430 + 762 Protein_70 hypothetical protein -
E4414_RS22625 71299..72177 - 879 Protein_71 galactose oxidase -
E4414_RS22630 72427..73500 + 1074 WP_162303305.1 IS110 family transposase -
E4414_RS22635 73539..73857 - 319 Protein_73 galactose oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..84267 84267
- flank IS/Tn - - 72427..73500 1073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

SpoVT-AbrB domain

(6-49)

Antitoxin

mRNA interferase PemK-like

(2-105)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>T123721 WP_000648189.1 NZ_CP039286:68652-68900 [Leptospira interrogans]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>T123721 NZ_CP039286:68652-68900 [Leptospira interrogans]
ATGAAGGCTTCAGTCGTAAAAATAGGCAATTCCAAAGGTATTCGAATTCCCAAGGCAGTCTTAGAAGAATGTCATATTGA
AGAAGAAGTAGATTTACTAATCGATAATAACAAATTAATTATTGTACCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAAACAATGTCATCTAATAAAGATGATAAATTATTTATTCCTGATTCAATAGATTTATTAGACAAGGATTGG
GAATGGTGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>AT123721 WP_000237066.1 NZ_CP039286:68894-69217 [Leptospira interrogans]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>AT123721 NZ_CP039286:68894-69217 [Leptospira interrogans]
ATGGTGATTTCTCAATACGAAGTATATCTAATAAATTTAGATCCAACGGTTGGACATGAGATAAAAAAATCAAGACCTTG
TGCAATAATCTCACCCAATGAAATGAATAAAACGATTGGAACAATTATTATTGCACCAATGACTACAAAATCTCGATCAT
ATCCGACAAGAGTTGAATTAACCTTTCAAGGAAAGAAGGGTTGGATTGTGTTAGATCAGATTCGAGCTGTTGATAAAACA
CGTCTGGTAAAGAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGGTAATAAAGGAAATGTTGGTTGA
TTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

References