Detailed information of TA system TA123720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 39001..39572
• Replicon: plasmid pLiSL2
• Accession: NZ_CP039285
• Organism: Leptospira interrogans strain FMAS_AW1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: E4414_RS21715
• Protein ID: WP_000240418.1
• Coordinates: 39001..39342 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: E4414_RS21720
• Protein ID: WP_000443825.1
• Coordinates: 39336..39572 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4414_RS21675	34827..35438	-	612	WP_017857624.1	hypothetical protein	-
E4414_RS21680	35546..36007	-	462	WP_000811790.1	hypothetical protein	-
E4414_RS21685	36530..36730	-	201	WP_017857623.1	hypothetical protein	-
E4414_RS21690	36735..36896	-	162	Protein_33	BrnT family toxin	-
E4414_RS23450	37045..37191	+	147	WP_167493406.1	hypothetical protein	-
E4414_RS21695	37154..37879	+	726	WP_000414903.1	hypothetical protein	-
E4414_RS21700	37882..38223	+	342	WP_000727287.1	hypothetical protein	-
E4414_RS21705	38284..38550	+	267	WP_000844450.1	hypothetical protein	-
E4414_RS21710	38550..38933	+	384	WP_000617796.1	hypothetical protein	-
E4414_RS21715	39001..39342	-	342	WP_000240418.1	endoribonuclease MazF	Toxin
E4414_RS21720	39336..39572	-	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
E4414_RS21725	39912..40919	-	1008	WP_000628975.1	hypothetical protein	-
E4414_RS21730	41255..41443	-	189	WP_000425065.1	hypothetical protein	-
E4414_RS21735	41613..41828	-	216	WP_017857593.1	hypothetical protein	-
E4414_RS21740	41818..42258	-	441	WP_001289702.1	hypothetical protein	-
E4414_RS21745	42255..42488	-	234	WP_000416314.1	hypothetical protein	-
E4414_RS21755	42693..43127	-	435	WP_061226524.1	M15 family metallopeptidase	-
E4414_RS21760	43127..43588	-	462	WP_137119371.1	hypothetical protein	-
E4414_RS21765	43595..44038	-	444	WP_001129679.1	hypothetical protein	-
E4414_RS21770	44038..44418	-	381	WP_045193734.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..129376	129376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T123720 WP_000240418.1 NZ_CP039285:c39342-39001 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T123720 NZ_CP039285:c39342-39001 [Leptospira interrogans]
TTGGTAAAGAATAGAAACTATACTCCTGAAAAAGGAGATATAGTGTGGTTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT123720 WP_000443825.1 NZ_CP039285:c39572-39336 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT123720 NZ_CP039285:c39572-39336 [Leptospira interrogans]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33