123719

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1328775..1329351 Replicon chromosome
Accession NZ_CP039283
Organism Leptospira interrogans strain FMAS_AW1

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag E4414_RS05630 Protein ID WP_000617906.1
Coordinates 1329010..1329351 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag E4414_RS05625 Protein ID WP_000844758.1
Coordinates 1328775..1329023 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4414_RS05610 1324427..1326409 + 1983 WP_000802799.1 HD family phosphohydrolase -
E4414_RS22975 1327051..1327509 - 459 WP_000453263.1 hypothetical protein -
E4414_RS05620 1327663..1327959 + 297 WP_000477061.1 helix-turn-helix domain-containing protein -
E4414_RS05625 1328775..1329023 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
E4414_RS05630 1329010..1329351 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E4414_RS05635 1330377..1331369 - 993 WP_000041092.1 aldolase -
E4414_RS05640 1331509..1332444 - 936 WP_000004530.1 ornithine carbamoyltransferase -
E4414_RS05650 1333248..1333715 + 468 WP_000060051.1 hypothetical protein -
E4414_RS05655 1333712..1334116 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T123719 WP_000617906.1 NZ_CP039283:1329010-1329351 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T123719 NZ_CP039283:1329010-1329351 [Leptospira interrogans]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTTTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT123719 WP_000844758.1 NZ_CP039283:1328775-1329023 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT123719 NZ_CP039283:1328775-1329023 [Leptospira interrogans]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References