Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 903149..903777 Replicon chromosome
Accession NZ_CP039283
Organism Leptospira interrogans strain FMAS_AW1

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag E4414_RS03850 Protein ID WP_000281042.1
Coordinates 903149..903547 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag E4414_RS03855 Protein ID WP_001192482.1
Coordinates 903547..903777 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4414_RS03825 898441..899208 + 768 WP_017857864.1 DUF1554 domain-containing protein -
E4414_RS03830 899749..900609 + 861 WP_000682231.1 hypothetical protein -
E4414_RS03835 900825..901283 - 459 WP_001239004.1 GNAT family N-acetyltransferase -
E4414_RS03840 901284..902360 - 1077 WP_001170569.1 hypothetical protein -
E4414_RS03845 902503..903051 - 549 WP_000479564.1 hypothetical protein -
E4414_RS03850 903149..903547 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
E4414_RS03855 903547..903777 - 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E4414_RS03860 904068..904547 - 480 WP_002108895.1 hypothetical protein -
E4414_RS03865 904584..904925 - 342 WP_000611885.1 hypothetical protein -
E4414_RS03870 905134..906024 - 891 WP_000789618.1 hypothetical protein -
E4414_RS03875 906061..907605 + 1545 WP_017857865.1 YceK/YidQ family lipoprotein -
E4414_RS03880 907931..908521 - 591 WP_000437520.1 energy transducer TonB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T123718 WP_000281042.1 NZ_CP039283:c903547-903149 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T123718 NZ_CP039283:c903547-903149 [Leptospira interrogans]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT123718 WP_001192482.1 NZ_CP039283:c903777-903547 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT123718 NZ_CP039283:c903777-903547 [Leptospira interrogans]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTCATTCAAAAAGTCGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAGCCGAAATCTGATAAAAGAGAAAAGCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References