Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2360322..2361297 Replicon chromosome
Accession NZ_CP039269
Organism Bacillus cereus strain MH19

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A2T7A9
Locus tag E4A35_RS12115 Protein ID WP_070172605.1
Coordinates 2360322..2361059 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag E4A35_RS12120 Protein ID WP_000588712.1
Coordinates 2361172..2361297 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4A35_RS12090 2355397..2356107 + 711 WP_128294920.1 class I SAM-dependent methyltransferase -
E4A35_RS12095 2356227..2356634 + 408 WP_000072479.1 VOC family protein -
E4A35_RS27655 2356740..2356832 + 93 Protein_2277 methyltransferase -
E4A35_RS12100 2356954..2358639 - 1686 WP_128294919.1 alpha-keto acid decarboxylase family protein -
E4A35_RS12105 2358746..2359228 + 483 WP_088016051.1 MarR family winged helix-turn-helix transcriptional regulator -
E4A35_RS12110 2359396..2360133 + 738 WP_000594156.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
E4A35_RS12115 2360322..2361059 + 738 WP_070172605.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
E4A35_RS12120 2361172..2361297 + 126 WP_000588712.1 hypothetical protein Antitoxin
E4A35_RS27485 2361374..2361550 + 177 WP_165443143.1 hypothetical protein -
E4A35_RS12125 2361594..2361869 - 276 WP_088016053.1 hypothetical protein -
E4A35_RS12130 2362150..2362387 - 238 Protein_2285 hypothetical protein -
E4A35_RS27490 2362723..2362899 + 177 WP_000808045.1 hypothetical protein -
E4A35_RS12135 2363043..2364512 + 1470 WP_070171971.1 beta-Ala-His dipeptidase -
E4A35_RS12140 2364747..2365217 - 471 WP_000670597.1 DUF5065 family protein -
E4A35_RS12145 2365421..2366014 + 594 WP_000265892.1 SGNH/GDSL hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28405.13 Da        Isoelectric Point: 8.2672

>T123717 WP_070172605.1 NZ_CP039269:2360322-2361059 [Bacillus cereus]
VISNIRIGLFILAIVFLILVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIMSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T123717 NZ_CP039269:2360322-2361059 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAATACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAATGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT123717 WP_000588712.1 NZ_CP039269:2361172-2361297 [Bacillus cereus]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT123717 NZ_CP039269:2361172-2361297 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A2T7A9

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References