Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/YoeB-RelB
Location 1897441..1897970 Replicon chromosome
Accession NZ_CP039266
Organism Lactobacillus crispatus strain DC21.1

Toxin (Protein)


Gene name Txe Uniprot ID A0A854ZB67
Locus tag E6A57_RS09440 Protein ID WP_025006274.1
Coordinates 1897441..1897698 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID V5E284
Locus tag E6A57_RS09445 Protein ID WP_013087009.1
Coordinates 1897695..1897970 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6A57_RS09415 1892775..1893590 + 816 WP_005720042.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
E6A57_RS09425 1894297..1895070 - 774 WP_158182227.1 XRE family transcriptional regulator -
E6A57_RS09430 1895299..1896591 - 1293 WP_148238714.1 transposase -
E6A57_RS09435 1896622..1897236 - 615 WP_013085784.1 IS607 family transposase -
E6A57_RS09440 1897441..1897698 - 258 WP_025006274.1 Txe/YoeB family addiction module toxin Toxin
E6A57_RS09445 1897695..1897970 - 276 WP_013087009.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
E6A57_RS09450 1898164..1899390 - 1227 WP_158182153.1 MFS transporter -
E6A57_RS09455 1899618..1901222 - 1605 WP_158182154.1 ABC transporter ATP-binding protein/permease -
E6A57_RS09460 1901417..1901827 + 411 WP_158182155.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
E6A57_RS09465 1901833..1902126 + 294 WP_080545719.1 type II toxin-antitoxin system RelE/ParE family toxin -
E6A57_RS09470 1902196..1902882 + 687 WP_150399414.1 cobalamin biosynthesis protein CobQ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10219.64 Da        Isoelectric Point: 8.9406

>T123715 WP_025006274.1 NZ_CP039266:c1897698-1897441 [Lactobacillus crispatus]
MIVAWTDDGWNDYVYWYDDGDYKKVSRINDLVKDMKRHPFTGIGKPEPLKRNLSGLWSRRIDSKNRIVYDCHKSMITIYS
CKDHY

Download         Length: 258 bp

>T123715 NZ_CP039266:c1897698-1897441 [Lactobacillus crispatus]
ATGATCGTAGCATGGACGGATGATGGTTGGAACGACTATGTGTATTGGTATGACGATGGTGATTATAAAAAGGTATCAAG
AATAAACGATCTTGTGAAAGATATGAAAAGACATCCATTTACAGGTATTGGTAAGCCTGAACCCTTAAAGAGAAATCTAT
CAGGGTTATGGTCAAGACGAATCGATTCGAAAAATCGGATCGTCTATGATTGTCATAAATCAATGATAACTATATATTCA
TGTAAGGATCACTATTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10597.82 Da        Isoelectric Point: 4.5716

>AT123715 WP_013087009.1 NZ_CP039266:c1897970-1897695 [Lactobacillus crispatus]
MDAVNYSNFRKNLKHYFKQVNDDSEPLIVTNKEPEDNVVVLGKEDYDALVETLEIQSNQYLMDKIERGRKQVSEGTIKQH
ELIDPEQVTKS

Download         Length: 276 bp

>AT123715 NZ_CP039266:c1897970-1897695 [Lactobacillus crispatus]
ATGGATGCGGTTAATTACAGTAATTTTCGTAAAAATTTAAAGCATTATTTTAAGCAAGTCAATGATGATAGTGAACCTTT
GATCGTAACTAATAAAGAACCAGAGGATAATGTGGTTGTCTTAGGTAAAGAAGATTATGATGCCTTGGTTGAAACATTAG
AAATTCAATCTAATCAATACCTAATGGACAAGATTGAAAGAGGGCGTAAACAAGTTAGTGAAGGCACGATAAAGCAACAT
GAACTAATTGATCCTGAGCAGGTAACAAAATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A854ZB67

Antitoxin

Source ID Structure
AlphaFold DB V5E284

References