Detailed information of TA system TA123713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 48998..49563
• Replicon: plasmid pLiLS1
• Accession: NZ_CP039260
• Organism: Leptospira interrogans strain FMAS_KW1

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0C5XAD8
• Locus tag: E4412_RS20485
• Protein ID: WP_000237066.1
• Coordinates: 48998..49321 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F6H5X2
• Locus tag: E4412_RS20490
• Protein ID: WP_000648189.1
• Coordinates: 49315..49563 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4412_RS20445	44370..44546	-	177	WP_137108934.1	PIN domain-containing protein	-
E4412_RS20450	44570..44806	-	237	WP_137108927.1	PIN domain-containing protein	-
E4412_RS20455	44809..45106	-	298	Protein_53	type II toxin-antitoxin system Phd/YefM family antitoxin	-
E4412_RS20460	45424..46830	-	1407	WP_137108928.1	IS5 family transposase	-
E4412_RS20465	47174..47515	-	342	WP_137108924.1	endoribonuclease MazF	-
E4412_RS20470	47509..47745	-	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
E4412_RS20475	48091..48513	-	423	WP_000402980.1	hypothetical protein	-
E4412_RS20480	48562..48774	-	213	WP_000447737.1	hypothetical protein	-
E4412_RS20485	48998..49321	-	324	WP_000237066.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E4412_RS20490	49315..49563	-	249	WP_000648189.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
E4412_RS20495	49778..50587	-	810	Protein_61	IS4 family transposase	-
E4412_RS20500	50698..51591	+	894	WP_000366212.1	IS982 family transposase	-
E4412_RS20505	51608..51817	-	210	WP_137108925.1	BrnT family toxin	-
E4412_RS20510	52245..53165	-	921	WP_137108926.1	DUF2971 domain-containing protein	-
E4412_RS21205	53591..53836	+	246	WP_061226370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..78201	78201

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12168.59 Da
• Isoelectric Point: 10.5161

>T123713 WP_000237066.1 NZ_CP039260:c49321-48998 [Leptospira interrogans]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Nucleotide

• Length: 324 bp

>T123713 NZ_CP039260:c49321-48998 [Leptospira interrogans]
ATGGTGATTTCTCAATACGAAGTATATCTAATAAATTTAGATCCAACGGTTGGACATGAGATAAAAAAATCAAGACCTTG
TGCAATAATCTCACCCAATGAAATGAATAAAACGATTGGAACAATTATTATTGCACCAATGACTACAAAATCTCGATCGT
ATCCGACAAGAGTTGAATTAACCTTTCAAGGAAAGAAGGGTTGGATTGTGTTAGATCAGATTCGAGCTGTCGATAAAACA
CGTCTGGTAAAGAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGGTAATAAAGGAAATGTTGGTTGA
TTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9507.04 Da
• Isoelectric Point: 5.1749

>AT123713 WP_000648189.1 NZ_CP039260:c49563-49315 [Leptospira interrogans]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Nucleotide

• Length: 249 bp

>AT123713 NZ_CP039260:c49563-49315 [Leptospira interrogans]
ATGAAGGCTTCAGTCGTAAAAATAGGCAATTCCAAAGGTATTCGAATTCCCAAGGCAGTCTTAGAAGAATGTCATATTGA
AGAAGAAGTAGATTTACTAATCGATAATAACAAATTAATTATTGTACCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAAACAATGTCATCTAATAAAGATGATAAATTATTTATTCCTGATTCAATAGATTTATTAGACAAGGATTGG
GAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A0C5XAD8

Antitoxin

• AlphaFold DB: A0A0F6H5X2