123710

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 29552..30117 Replicon plasmid pLiLS1
Accession NZ_CP039260
Organism Leptospira interrogans strain FMAS_KW1

Toxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag E4412_RS20360 Protein ID WP_000237066.1
Coordinates 29552..29875 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag E4412_RS20365 Protein ID WP_000648189.1
Coordinates 29869..30117 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4412_RS20325 24910..25356 - 447 WP_017857748.1 PIN domain-containing protein -
E4412_RS20330 25359..25658 - 300 WP_000835631.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
E4412_RS20335 26074..27384 - 1311 WP_174978845.1 IS5 family transposase -
E4412_RS20340 27728..28069 - 342 WP_137108924.1 endoribonuclease MazF -
E4412_RS20345 28063..28299 - 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
E4412_RS20350 28645..29067 - 423 WP_000402980.1 hypothetical protein -
E4412_RS20355 29116..29328 - 213 WP_000447737.1 hypothetical protein -
E4412_RS20360 29552..29875 - 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E4412_RS20365 29869..30117 - 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E4412_RS20370 30332..31141 - 810 Protein_38 IS4 family transposase -
E4412_RS20375 31252..32145 + 894 WP_000366212.1 IS982 family transposase -
E4412_RS20380 32162..32371 - 210 WP_137108925.1 BrnT family toxin -
E4412_RS20385 32799..33719 - 921 WP_137108926.1 DUF2971 domain-containing protein -
E4412_RS21190 34144..34374 + 231 WP_174978842.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..78201 78201


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-105)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>T123710 WP_000237066.1 NZ_CP039260:c29875-29552 [Leptospira interrogans]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>T123710 NZ_CP039260:c29875-29552 [Leptospira interrogans]
ATGGTGATTTCTCAATACGAAGTATATCTAATAAATTTAGATCCAACGGTTGGACATGAGATAAAAAAATCAAGACCTTG
TGCAATAATCTCACCCAATGAAATGAATAAAACGATTGGAACAATTATTATTGCACCAATGACTACAAAATCTCGATCGT
ATCCGACAAGAGTTGAATTAACCTTTCAAGGAAAGAAGGGTTGGATTGTGTTAGATCAGATTCGAGCTGTCGATAAAACA
CGTCTGGTAAAGAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGGTAATAAAGGAAATGTTGGTTGA
TTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>AT123710 WP_000648189.1 NZ_CP039260:c30117-29869 [Leptospira interrogans]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>AT123710 NZ_CP039260:c30117-29869 [Leptospira interrogans]
ATGAAGGCTTCAGTCGTAAAAATAGGCAATTCCAAAGGTATTCGAATTCCCAAGGCAGTCTTAGAAGAATGTCATATTGA
AGAAGAAGTAGATTTACTAATCGATAATAACAAATTAATTATTGTACCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAAACAATGTCATCTAATAAAGATGATAAATTATTTATTCCTGATTCAATAGATTTATTAGACAAGGATTGG
GAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

References