Detailed information of TA system TA123709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 27728..28299
• Replicon: plasmid pLiLS1
• Accession: NZ_CP039260
• Organism: Leptospira interrogans strain FMAS_KW1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E4412_RS20340
• Protein ID: WP_137108924.1
• Coordinates: 27728..28069 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: E4412_RS20345
• Protein ID: WP_000443825.1
• Coordinates: 28063..28299 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4412_RS20305	23435..23674	-	240	WP_000833998.1	antitoxin	-
E4412_RS20310	23664..23900	-	237	WP_137108922.1	nucleotidyltransferase domain-containing protein	-
E4412_RS20320	24295..24618	-	324	WP_017857747.1	hypothetical protein	-
E4412_RS20325	24910..25356	-	447	WP_017857748.1	PIN domain-containing protein	-
E4412_RS20330	25359..25658	-	300	WP_000835631.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
E4412_RS20335	26074..27384	-	1311	WP_174978845.1	IS5 family transposase	-
E4412_RS20340	27728..28069	-	342	WP_137108924.1	endoribonuclease MazF	Toxin
E4412_RS20345	28063..28299	-	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
E4412_RS20350	28645..29067	-	423	WP_000402980.1	hypothetical protein	-
E4412_RS20355	29116..29328	-	213	WP_000447737.1	hypothetical protein	-
E4412_RS20360	29552..29875	-	324	WP_000237066.1	type II toxin-antitoxin system PemK/MazF family toxin	-
E4412_RS20365	29869..30117	-	249	WP_000648189.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
E4412_RS20370	30332..31141	-	810	Protein_38	IS4 family transposase	-
E4412_RS20375	31252..32145	+	894	WP_000366212.1	IS982 family transposase	-
E4412_RS20380	32162..32371	-	210	WP_137108925.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..78201	78201

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12975.22 Da
• Isoelectric Point: 10.1024

>T123709 WP_137108924.1 NZ_CP039260:c28069-27728 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKDYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAKFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T123709 NZ_CP039260:c28069-27728 [Leptospira interrogans]
TTGGTAAAGAATAGAAACTATACTCCTGAAAAAGGAGATATAGTGTGGCTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGATTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAAAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT123709 WP_000443825.1 NZ_CP039260:c28299-28063 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT123709 NZ_CP039260:c28299-28063 [Leptospira interrogans]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F6HC33