123708

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 2805423..2805999 Replicon chromosome
Accession NZ_CP039258
Organism Leptospira interrogans strain FMAS_KW1

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag E4412_RS12370 Protein ID WP_000617906.1
Coordinates 2805423..2805764 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag E4412_RS12375 Protein ID WP_000844758.1
Coordinates 2805751..2805999 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4412_RS12350 2800679..2801083 - 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -
E4412_RS12355 2801080..2801547 - 468 WP_000060053.1 hypothetical protein -
E4412_RS12360 2802331..2803266 + 936 WP_000004530.1 ornithine carbamoyltransferase -
E4412_RS12365 2803406..2804398 + 993 WP_137108480.1 CoA ester lyase -
E4412_RS12370 2805423..2805764 - 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E4412_RS12375 2805751..2805999 - 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
E4412_RS12380 2806816..2807112 - 297 WP_000477061.1 helix-turn-helix domain-containing protein -
E4412_RS21050 2807266..2807724 + 459 WP_174978806.1 hypothetical protein -
E4412_RS12390 2808365..2810347 - 1983 WP_137108481.1 HD family phosphohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T123708 WP_000617906.1 NZ_CP039258:c2805764-2805423 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T123708 NZ_CP039258:c2805764-2805423 [Leptospira interrogans]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTTTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT123708 WP_000844758.1 NZ_CP039258:c2805999-2805751 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT123708 NZ_CP039258:c2805999-2805751 [Leptospira interrogans]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References