Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1448938..1449514 Replicon chromosome
Accession NZ_CP039256
Organism Leptospira interrogans strain FMAS_KW2

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag E4413_RS06255 Protein ID WP_000617906.1
Coordinates 1449173..1449514 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag E4413_RS06250 Protein ID WP_000844758.1
Coordinates 1448938..1449186 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4413_RS06235 1444590..1446572 + 1983 WP_137170961.1 HD family phosphohydrolase -
E4413_RS20585 1447213..1447671 - 459 Protein_1149 hypothetical protein -
E4413_RS06245 1447825..1448121 + 297 WP_000477061.1 helix-turn-helix domain-containing protein -
E4413_RS06250 1448938..1449186 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
E4413_RS06255 1449173..1449514 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E4413_RS06260 1450539..1451531 - 993 WP_137108480.1 CoA ester lyase -
E4413_RS06265 1451671..1452606 - 936 WP_000004530.1 ornithine carbamoyltransferase -
E4413_RS06270 1453390..1453857 + 468 WP_000060053.1 hypothetical protein -
E4413_RS06275 1453854..1454258 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T123707 WP_000617906.1 NZ_CP039256:1449173-1449514 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T123707 NZ_CP039256:1449173-1449514 [Leptospira interrogans]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTTTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT123707 WP_000844758.1 NZ_CP039256:1448938-1449186 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT123707 NZ_CP039256:1448938-1449186 [Leptospira interrogans]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References