Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 1029872..1030500 Replicon chromosome
Accession NZ_CP039256
Organism Leptospira interrogans strain FMAS_KW2

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag E4413_RS04535 Protein ID WP_000281042.1
Coordinates 1029872..1030270 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag E4413_RS04540 Protein ID WP_001192482.1
Coordinates 1030270..1030500 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4413_RS04515 1025606..1026373 + 768 WP_137108560.1 DUF1554 domain-containing protein -
E4413_RS04520 1026914..1027756 + 843 WP_137170910.1 hypothetical protein -
E4413_RS04525 1028012..1029082 - 1071 Protein_842 hypothetical protein -
E4413_RS04530 1029193..1029774 - 582 WP_137170911.1 hypothetical protein -
E4413_RS04535 1029872..1030270 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
E4413_RS04540 1030270..1030500 - 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E4413_RS04545 1031098..1031679 - 582 WP_137170912.1 hypothetical protein -
E4413_RS04555 1031919..1033067 - 1149 WP_137170914.1 hypothetical protein -
E4413_RS04560 1033252..1034367 - 1116 WP_000353261.1 protein kinase -
E4413_RS04565 1034422..1034775 - 354 WP_137108558.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T123706 WP_000281042.1 NZ_CP039256:c1030270-1029872 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T123706 NZ_CP039256:c1030270-1029872 [Leptospira interrogans]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT123706 WP_001192482.1 NZ_CP039256:c1030500-1030270 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT123706 NZ_CP039256:c1030500-1030270 [Leptospira interrogans]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTTATCCAAAAAGTCGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAGCCGAAATCTGATAAAAGAGAAAAGCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References