Detailed information of TA system TA123700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 31065..31708
• Replicon: plasmid unnamed5
• Accession: NZ_CP039245
• Organism: Piscirickettsia salmonis strain MR5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: PsalMR5_RS24180
• Protein ID: WP_016210974.1
• Coordinates: 31065..31472 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1L6TI10
• Locus tag: PsalMR5_RS24185
• Protein ID: WP_027242932.1
• Coordinates: 31481..31708 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalMR5_RS24155	26530..27771	-	1242	WP_155081941.1	phage portal protein	-
PsalMR5_RS24160	27768..28745	-	978	WP_155081942.1	terminase large subunit	-
PsalMR5_RS24165	28783..29757	-	975	WP_155079741.1	IS30 family transposase	-
PsalMR5_RS24170	29817..30047	-	231	WP_155081943.1	hypothetical protein	-
PsalMR5_RS24175	30268..31053	-	786	WP_155081944.1	DNA/RNA non-specific endonuclease	-
PsalMR5_RS24180	31065..31472	-	408	WP_016210974.1	DUF4411 family protein	Toxin
PsalMR5_RS24185	31481..31708	-	228	WP_027242932.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PsalMR5_RS24190	31856..32221	-	366	WP_155081945.1	hypothetical protein	-
PsalMR5_RS24195	32370..32840	-	471	WP_155081946.1	single-stranded DNA-binding protein	-
PsalMR5_RS24200	32844..33074	-	231	WP_155081947.1	hypothetical protein	-
PsalMR5_RS24205	33321..34295	-	975	WP_155081450.1	IS30 family transposase	-
PsalMR5_RS25115	34340..34477	+	138	WP_196215084.1	hypothetical protein	-
PsalMR5_RS24215	34518..34856	-	339	WP_155081949.1	hypothetical protein	-
PsalMR5_RS24220	35091..35648	-	558	WP_155079478.1	IS630 family transposase	-
PsalMR5_RS24225	35632..36018	-	387	WP_155079476.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..50242	50242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T123700 WP_016210974.1 NZ_CP039245:c31472-31065 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T123700 NZ_CP039245:c31472-31065 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCTGTTCTAGTAACGCATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8948.07 Da
• Isoelectric Point: 4.5700

>AT123700 WP_027242932.1 NZ_CP039245:c31708-31481 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDRQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123700 NZ_CP039245:c31708-31481 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCACATGC
CGGATGTATCGGATGATTTTATGGAAGATCGCCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: A0A1L6TI10