Detailed information of TA system TA123697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 41298..41868
• Replicon: plasmid unnamed2
• Accession: NZ_CP039242
• Organism: Piscirickettsia salmonis strain MR5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: PsalMR5_RS22400
• Protein ID: WP_155081830.1
• Coordinates: 41542..41868 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PsalMR5_RS22395
• Protein ID: WP_122943070.1
• Coordinates: 41298..41540 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalMR5_RS22355	36959..37495	-	537	WP_196215040.1	transposase	-
PsalMR5_RS22360	37502..38059	-	558	WP_155079478.1	IS630 family transposase	-
PsalMR5_RS22365	38043..38429	-	387	WP_155079476.1	helix-turn-helix domain-containing protein	-
PsalMR5_RS25055	38426..38593	-	168	WP_196215059.1	transposase zinc-binding domain-containing protein	-
PsalMR5_RS22370	38912..39103	-	192	Protein_55	IS30 family transposase	-
PsalMR5_RS22375	39272..40003	-	732	WP_155081754.1	IS30 family transposase	-
PsalMR5_RS22380	40061..40513	-	453	WP_155068622.1	IS630 family transposase	-
PsalMR5_RS22385	40545..40895	-	351	WP_155066936.1	IS630 transposase-related protein	-
PsalMR5_RS22390	40968..41090	-	123	Protein_59	helix-turn-helix domain-containing protein	-
PsalMR5_RS22395	41298..41540	+	243	WP_122943070.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PsalMR5_RS22400	41542..41868	+	327	WP_155081830.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PsalMR5_RS22405	42035..42181	-	147	WP_155080251.1	hypothetical protein	-
PsalMR5_RS25060	43088..43255	+	168	WP_155067835.1	hypothetical protein	-
PsalMR5_RS22415	43201..43776	+	576	WP_155066610.1	IS630 family transposase	-
PsalMR5_RS22420	43780..44799	-	1020	WP_155080567.1	IS91 family transposase	-
PsalMR5_RS22425	45003..45675	+	673	Protein_66	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..158411	158411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11917.80 Da
• Isoelectric Point: 10.1365

>T123697 WP_155081830.1 NZ_CP039242:41542-41868 [Piscirickettsia salmonis]
VPARGDIIFLSLDPTLGSEQKGNRPFLVLTSKPYNSKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARDWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 327 bp

>T123697 NZ_CP039242:41542-41868 [Piscirickettsia salmonis]
GTGCCTGCGCGCGGTGATATTATTTTTCTTAGCCTAGACCCTACGTTAGGGAGTGAACAAAAAGGAAACCGGCCTTTTTT
AGTGTTAACTTCTAAGCCTTATAACTCAAAGGTTGGACTTGTGACGGGATGTCCTGTTACTTCAAAGATTAAAGGGTATC
CTTTTGAAGTTGAGTTACCTAAAAACAATAGTGTTCACGGTGCTGTACTTGTCAACCAACTTACAACAAAAGACTTTCGT
GCTAGGGACTGGAATCATGCTGATAAACTCGATAAAAAATCTCTTGAAAGAGTTCAGGGGTTAATTGATGTAATGTTAAA
AGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8936.07 Da
• Isoelectric Point: 4.4625

>AT123697 WP_122943070.1 NZ_CP039242:41298-41540 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEDLGGERFE

Nucleotide

• Length: 243 bp

>AT123697 NZ_CP039242:41298-41540 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGTAATAGTTTGGGTTTATTGTTGCCATTGAGCATCGCGCGCGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATCAAACCTTTGACACATACGCAGACCCTAGAAT
TATTATTATCAGAAATCACTCCTGAAAACAGCCATGATTTAGTAGACTGGGGTGAAGATTTAGGAGGAGAGCGCTTTGAA
TAA