Detailed information of TA system TA123693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 64046..64616
• Replicon: plasmid unnamed2
• Accession: NZ_CP039236
• Organism: Piscirickettsia salmonis strain BI1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: PsalBI1_RS22345
• Protein ID: WP_155081830.1
• Coordinates: 64046..64372 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PsalBI1_RS22350
• Protein ID: WP_122943070.1
• Coordinates: 64374..64616 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalBI1_RS22320	60239..60911	-	673	Protein_83	IS6 family transposase	-
PsalBI1_RS22325	61115..62134	+	1020	WP_155080567.1	IS91 family transposase	-
PsalBI1_RS22330	62138..62713	-	576	WP_155066610.1	IS630 family transposase	-
PsalBI1_RS24540	62659..62826	-	168	WP_155067835.1	hypothetical protein	-
PsalBI1_RS22340	63733..63879	+	147	WP_155080251.1	hypothetical protein	-
PsalBI1_RS22345	64046..64372	-	327	WP_155081830.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PsalBI1_RS22350	64374..64616	-	243	WP_122943070.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PsalBI1_RS22355	64824..64946	+	123	Protein_90	helix-turn-helix domain-containing protein	-
PsalBI1_RS22360	65019..65369	+	351	WP_155066936.1	IS630 transposase-related protein	-
PsalBI1_RS22365	65401..65853	+	453	WP_155068622.1	IS630 family transposase	-
PsalBI1_RS22370	65866..66714	+	849	Protein_93	IS30 family transposase	-
PsalBI1_RS24545	67033..67200	+	168	WP_196215059.1	transposase zinc-binding domain-containing protein	-
PsalBI1_RS22375	67197..67583	+	387	WP_155079476.1	helix-turn-helix domain-containing protein	-
PsalBI1_RS22380	67567..68124	+	558	WP_155079478.1	IS630 family transposase	-
PsalBI1_RS22385	68131..68667	+	537	WP_196215040.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..156158	156158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11917.80 Da
• Isoelectric Point: 10.1365

>T123693 WP_155081830.1 NZ_CP039236:c64372-64046 [Piscirickettsia salmonis]
VPARGDIIFLSLDPTLGSEQKGNRPFLVLTSKPYNSKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARDWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 327 bp

>T123693 NZ_CP039236:c64372-64046 [Piscirickettsia salmonis]
GTGCCTGCGCGCGGTGATATTATTTTTCTTAGCCTAGACCCTACGTTAGGGAGTGAACAAAAAGGAAACCGGCCTTTTTT
AGTGTTAACTTCTAAGCCTTATAACTCAAAGGTTGGACTTGTGACGGGATGTCCTGTTACTTCAAAGATTAAAGGGTATC
CTTTTGAAGTTGAGTTACCTAAAAACAATAGTGTTCACGGTGCTGTACTTGTCAACCAACTTACAACAAAAGACTTTCGT
GCTAGGGACTGGAATCATGCTGATAAACTCGATAAAAAATCTCTTGAAAGAGTTCAGGGGTTAATTGATGTAATGTTAAA
AGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8936.07 Da
• Isoelectric Point: 4.4625

>AT123693 WP_122943070.1 NZ_CP039236:c64616-64374 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEDLGGERFE

Nucleotide

• Length: 243 bp

>AT123693 NZ_CP039236:c64616-64374 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGTAATAGTTTGGGTTTATTGTTGCCATTGAGCATCGCGCGCGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATCAAACCTTTGACACATACGCAGACCCTAGAAT
TATTATTATCAGAAATCACTCCTGAAAACAGCCATGATTTAGTAGACTGGGGTGAAGATTTAGGAGGAGAGCGCTTTGAA
TAA