Detailed information of TA system TA123689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 57501..58071
• Replicon: plasmid unnamed2
• Accession: NZ_CP039229
• Organism: Piscirickettsia salmonis strain SR1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: PsalSR1_RS22575
• Protein ID: WP_155081830.1
• Coordinates: 57501..57827 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PsalSR1_RS22580
• Protein ID: WP_122943070.1
• Coordinates: 57829..58071 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalSR1_RS22550	53694..54366	-	673	Protein_72	IS6 family transposase	-
PsalSR1_RS22555	54570..55589	+	1020	WP_155080567.1	IS91 family transposase	-
PsalSR1_RS22560	55593..56168	-	576	WP_155066610.1	IS630 family transposase	-
PsalSR1_RS25565	56114..56281	-	168	WP_155067835.1	hypothetical protein	-
PsalSR1_RS22570	57188..57334	+	147	WP_155080251.1	hypothetical protein	-
PsalSR1_RS22575	57501..57827	-	327	WP_155081830.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PsalSR1_RS22580	57829..58071	-	243	WP_122943070.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PsalSR1_RS22585	58279..58401	+	123	Protein_79	helix-turn-helix domain-containing protein	-
PsalSR1_RS22590	58474..58824	+	351	WP_155066936.1	IS630 transposase-related protein	-
PsalSR1_RS22595	58856..59308	+	453	WP_155068622.1	IS630 family transposase	-
PsalSR1_RS22600	59366..60097	+	732	WP_155081754.1	IS30 family transposase	-
PsalSR1_RS22605	60266..60457	+	192	Protein_83	IS30 family transposase	-
PsalSR1_RS25570	60776..60943	+	168	WP_196215059.1	transposase zinc-binding domain-containing protein	-
PsalSR1_RS22610	60940..61326	+	387	WP_155079476.1	helix-turn-helix domain-containing protein	-
PsalSR1_RS22615	61310..61867	+	558	WP_155079478.1	IS630 family transposase	-
PsalSR1_RS22620	61874..62410	+	537	WP_196215040.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..167241	167241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11917.80 Da
• Isoelectric Point: 10.1365

>T123689 WP_155081830.1 NZ_CP039229:c57827-57501 [Piscirickettsia salmonis]
VPARGDIIFLSLDPTLGSEQKGNRPFLVLTSKPYNSKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARDWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 327 bp

>T123689 NZ_CP039229:c57827-57501 [Piscirickettsia salmonis]
GTGCCTGCGCGCGGTGATATTATTTTTCTTAGCCTAGACCCTACGTTAGGGAGTGAACAAAAAGGAAACCGGCCTTTTTT
AGTGTTAACTTCTAAGCCTTATAACTCAAAGGTTGGACTTGTGACGGGATGTCCTGTTACTTCAAAGATTAAAGGGTATC
CTTTTGAAGTTGAGTTACCTAAAAACAATAGTGTTCACGGTGCTGTACTTGTCAACCAACTTACAACAAAAGACTTTCGT
GCTAGGGACTGGAATCATGCTGATAAACTCGATAAAAAATCTCTTGAAAGAGTTCAGGGGTTAATTGATGTAATGTTAAA
AGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8936.07 Da
• Isoelectric Point: 4.4625

>AT123689 WP_122943070.1 NZ_CP039229:c58071-57829 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEDLGGERFE

Nucleotide

• Length: 243 bp

>AT123689 NZ_CP039229:c58071-57829 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGTAATAGTTTGGGTTTATTGTTGCCATTGAGCATCGCGCGCGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATCAAACCTTTGACACATACGCAGACCCTAGAAT
TATTATTATCAGAAATCACTCCTGAAAACAGCCATGATTTAGTAGACTGGGGTGAAGATTTAGGAGGAGAGCGCTTTGAA
TAA