Detailed information of TA system TA123688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 173269..173912
• Replicon: plasmid unnamed1
• Accession: NZ_CP039228
• Organism: Piscirickettsia salmonis strain SR1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: PsalSR1_RS21900
• Protein ID: WP_016210974.1
• Coordinates: 173505..173912 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1L6TI10
• Locus tag: PsalSR1_RS21895
• Protein ID: WP_027242932.1
• Coordinates: 173269..173496 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalSR1_RS21855	168959..169345	+	387	WP_155079476.1	helix-turn-helix domain-containing protein	-
PsalSR1_RS21860	169329..169886	+	558	WP_155079478.1	IS630 family transposase	-
PsalSR1_RS21865	170121..170459	+	339	WP_155081949.1	hypothetical protein	-
PsalSR1_RS21870	170452..170622	+	171	WP_155081948.1	hypothetical protein	-
PsalSR1_RS21875	170682..171656	+	975	WP_155081450.1	IS30 family transposase	-
PsalSR1_RS21880	171903..172133	+	231	WP_155081947.1	hypothetical protein	-
PsalSR1_RS21885	172137..172607	+	471	WP_155081946.1	single-stranded DNA-binding protein	-
PsalSR1_RS21890	172756..173121	+	366	WP_155081945.1	hypothetical protein	-
PsalSR1_RS21895	173269..173496	+	228	WP_027242932.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PsalSR1_RS21900	173505..173912	+	408	WP_016210974.1	DUF4411 family protein	Toxin
PsalSR1_RS21905	173924..174709	+	786	WP_155081944.1	DNA/RNA non-specific endonuclease	-
PsalSR1_RS21910	174930..175160	+	231	WP_155081943.1	hypothetical protein	-
PsalSR1_RS21915	175220..176194	+	975	WP_155079741.1	IS30 family transposase	-
PsalSR1_RS21920	176232..177425	+	1194	WP_155081938.1	IS91 family transposase	-
PsalSR1_RS21925	177619..178428	+	810	WP_196215089.1	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..215583	215583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T123688 WP_016210974.1 NZ_CP039228:173505-173912 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T123688 NZ_CP039228:173505-173912 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCTGTTCTAGTAACGCATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8948.07 Da
• Isoelectric Point: 4.5700

>AT123688 WP_027242932.1 NZ_CP039228:173269-173496 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDRQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123688 NZ_CP039228:173269-173496 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCACATGC
CGGATGTATCGGATGATTTTATGGAAGATCGCCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: A0A1L6TI10