Detailed information of TA system TA123687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2382..2988
• Replicon: plasmid unnamed7
• Accession: NZ_CP039226
• Organism: Piscirickettsia salmonis strain NVI 5692

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PsalN5692_RS20445
• Protein ID: WP_196215566.1
• Coordinates: 2382..2642 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PsalN5692_RS20450
• Protein ID: WP_155069146.1
• Coordinates: 2635..2988 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalN5692_RS20430	1..801	+	801	WP_155069142.1	ParA family protein	-
PsalN5692_RS20435	809..1291	+	483	WP_155069143.1	hypothetical protein	-
PsalN5692_RS20440	1583..2173	-	591	WP_155069144.1	site-specific integrase	-
PsalN5692_RS20445	2382..2642	+	261	WP_196215566.1	type II toxin-antitoxin system HicA family toxin	Toxin
PsalN5692_RS20450	2635..2988	+	354	WP_155069146.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PsalN5692_RS20455	3147..3887	+	741	WP_196215567.1	class I SAM-dependent methyltransferase	-
PsalN5692_RS20460	3892..4347	+	456	WP_155069147.1	hypothetical protein	-
PsalN5692_RS20465	4694..5407	+	714	WP_155069148.1	queuosine precursor transporter	-
PsalN5692_RS20470	5515..6351	+	837	WP_155069149.1	IS30 family transposase	-
PsalN5692_RS20475	6388..7005	+	618	WP_155069150.1	IS6 family transposase	-
PsalN5692_RS20480	7164..7475	-	312	WP_155069151.1	head-tail connector protein	-
PsalN5692_RS20990	7472..7627	-	156	WP_196215568.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..19892	19892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9688.28 Da
• Isoelectric Point: 10.7082

>T123687 WP_196215566.1 NZ_CP039226:2382-2642 [Piscirickettsia salmonis]
MNNKHRKTLKDIFHNPIKASIVWSDIEKLLLALEAEVVERDGSRVAVILNGVKTTFHRPHPEKTTDKGAVKSIRMFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T123687 NZ_CP039226:2382-2642 [Piscirickettsia salmonis]
ATGAACAATAAACATCGAAAGACTCTAAAAGACATTTTTCATAATCCGATCAAGGCTAGTATTGTTTGGTCTGATATAGA
AAAACTTTTGCTAGCGCTAGAAGCTGAAGTGGTGGAGCGCGATGGCTCAAGAGTCGCGGTGATTTTAAATGGAGTAAAAA
CAACATTTCATAGACCTCACCCGGAAAAAACAACGGATAAAGGGGCAGTGAAGTCGATTAGGATGTTTTTAAAGAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13464.38 Da
• Isoelectric Point: 5.0200

>AT123687 WP_155069146.1 NZ_CP039226:2635-2988 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGVVPNIKDIISFRGKNIKELTKDFKLAIDDYLALCDERGEEADKPFSGRFMTRLGSDL
HEEVFLASMNDSEVKSMNDWVVSACLEKLKHDQAVKR

Nucleotide

• Length: 354 bp

>AT123687 NZ_CP039226:2635-2988 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATAGGTAAAGTTGAAGAAATTCACGAAGGCGATGAATATATTTATGGAGTTGTGCCAAA
TATAAAAGATATTATTTCTTTCAGAGGTAAGAATATAAAAGAACTAACAAAGGATTTTAAGTTAGCAATTGATGATTATT
TGGCTTTATGCGATGAGCGTGGAGAAGAAGCAGATAAGCCATTTTCAGGTAGATTTATGACTAGATTAGGCAGTGACTTG
CATGAAGAGGTATTTTTAGCTTCTATGAATGACTCTGAAGTAAAATCGATGAATGATTGGGTTGTGTCCGCGTGCTTGGA
AAAACTGAAGCATGACCAAGCTGTTAAACGATAA