Detailed information of TA system TA123680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 51084..51727
• Replicon: plasmid unnamed2
• Accession: NZ_CP039216
• Organism: Piscirickettsia salmonis strain Psal-104a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: Psal104a_RS17895
• Protein ID: WP_016210974.1
• Coordinates: 51320..51727 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: Psal104a_RS17890
• Protein ID: WP_016210972.1
• Coordinates: 51084..51311 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal104a_RS17835	46180..46485	+	306	WP_016210958.1	hypothetical protein	-
Psal104a_RS17840	46639..46938	+	300	WP_016210976.1	hypothetical protein	-
Psal104a_RS17845	46985..47800	+	816	WP_016210962.1	hypothetical protein	-
Psal104a_RS17850	47823..48383	+	561	WP_016210973.1	hypothetical protein	-
Psal104a_RS17855	48352..48714	+	363	WP_016210959.1	hypothetical protein	-
Psal104a_RS17860	48707..49054	+	348	WP_016210966.1	hypothetical protein	-
Psal104a_RS17865	49051..49290	+	240	WP_016210963.1	hypothetical protein	-
Psal104a_RS17870	49386..49673	+	288	WP_036794065.1	hypothetical protein	-
Psal104a_RS17875	49670..49954	+	285	WP_036794070.1	hypothetical protein	-
Psal104a_RS17880	49956..50429	+	474	WP_016210960.1	single-stranded DNA-binding protein	-
Psal104a_RS17885	50544..50936	+	393	WP_129556726.1	hypothetical protein	-
Psal104a_RS17890	51084..51311	+	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Psal104a_RS17895	51320..51727	+	408	WP_016210974.1	DUF4411 family protein	Toxin
Psal104a_RS17900	51844..52524	+	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
Psal104a_RS17905	52703..52996	+	294	WP_016210977.1	zinc ribbon domain-containing protein	-
Psal104a_RS17910	53214..53396	-	183	WP_129556724.1	hypothetical protein	-
Psal104a_RS17915	53541..53720	-	180	WP_155046773.1	phosphatase	-
Psal104a_RS17920	53815..54132	+	318	WP_129556723.1	hypothetical protein	-
Psal104a_RS17925	54433..54816	+	384	WP_032126134.1	HNH endonuclease	-
Psal104a_RS17930	54904..55383	+	480	WP_016212234.1	P27 family phage terminase small subunit	-
Psal104a_RS17935	55387..55596	+	210	WP_016212231.1	hypothetical protein	-
Psal104a_RS17940	55651..55968	+	318	WP_164997282.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..79943	79943

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T123680 WP_016210974.1 NZ_CP039216:51320-51727 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T123680 NZ_CP039216:51320-51727 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT123680 WP_016210972.1 NZ_CP039216:51084-51311 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123680 NZ_CP039216:51084-51311 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5