Detailed information of TA system TA123665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 38934..39504
• Replicon: plasmid unnamed1
• Accession: NZ_CP039202
• Organism: Piscirickettsia salmonis strain Psal-163

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Psal163_RS15830
• Protein ID: WP_036781349.1
• Coordinates: 38934..39260 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Psal163_RS15835
• Protein ID: WP_016212365.1
• Coordinates: 39262..39504 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal163_RS15795	34610..34987	+	378	Protein_41	DDE-type integrase/transposase/recombinase	-
Psal163_RS15800	35088..35867	+	780	WP_081377351.1	transposase	-
Psal163_RS15810	36056..36388	+	333	WP_032126739.1	transposase	-
Psal163_RS15815	36553..36930	+	378	WP_016212154.1	DDE-type integrase/transposase/recombinase	-
Psal163_RS15820	37237..37620	-	384	WP_016212152.1	hypothetical protein	-
Psal163_RS15825	38090..38818	-	729	WP_075273842.1	IS6 family transposase	-
Psal163_RS15830	38934..39260	-	327	WP_036781349.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Psal163_RS15835	39262..39504	-	243	WP_016212365.1	antidote-toxin recognition MazE family protein	Antitoxin
Psal163_RS15840	39709..40149	+	441	Protein_49	tyrosine-type recombinase/integrase	-
Psal163_RS15845	40217..40684	+	468	Protein_50	IS6-like element ISPsa2 family transposase	-
Psal163_RS15850	40858..41037	-	180	WP_155047017.1	hypothetical protein	-
Psal163_RS15855	40964..41233	+	270	Protein_52	DDE-type integrase/transposase/recombinase	-
Psal163_RS15860	41516..43150	+	1635	WP_075273844.1	protein kinase	-
Psal163_RS17320	43271..44002	+	732	WP_016212311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..158203	158203
	-	inside	IScluster/Tn	-	-	29602..44890	15288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11971.89 Da
• Isoelectric Point: 10.1733

>T123665 WP_036781349.1 NZ_CP039202:c39260-38934 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARGWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 327 bp

>T123665 NZ_CP039202:c39260-38934 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTGACTTCCAAGCCCTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGAGCAGTGTTGGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGGCTGGAATCATGCTGATAAGCTCGATAAAAAATCTCTTGAAAGAGTTCAGGGGCTAATTGATGTGATGTTGAA
AGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.09 Da
• Isoelectric Point: 4.4750

>AT123665 WP_016212365.1 NZ_CP039202:c39504-39262 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT123665 NZ_CP039202:c39504-39262 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGGAATAGCTTGGGGTTGTTGCTACCCTTGAGCATCGCACGTGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACTCATACGCAGACCCTTGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA