Detailed information of TA system TA123664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4014..4620
• Replicon: plasmid unnamed5
• Accession: NZ_CP039200
• Organism: Piscirickettsia salmonis strain Psal-161

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: Psal161_RS17555
• Protein ID: WP_027242955.1
• Coordinates: 4014..4274 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: Psal161_RS17560
• Protein ID: WP_054300685.1
• Coordinates: 4267..4620 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal161_RS17530	1..993	+	993	WP_054300689.1	ParA family protein	-
Psal161_RS17535	1010..2377	+	1368	WP_054300688.1	hypothetical protein	-
Psal161_RS17540	2741..2995	+	255	WP_054300687.1	DUF4160 domain-containing protein	-
Psal161_RS17545	3038..3271	+	234	WP_036781101.1	hypothetical protein	-
Psal161_RS17550	3318..3752	+	435	WP_054300686.1	hypothetical protein	-
Psal161_RS17555	4014..4274	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
Psal161_RS17560	4267..4620	+	354	WP_054300685.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
Psal161_RS17565	4724..5524	+	801	WP_054300684.1	methyltransferase domain-containing protein	-
Psal161_RS17570	5521..6333	-	813	WP_016211068.1	helix-turn-helix transcriptional regulator	-
Psal161_RS17575	6337..7578	-	1242	WP_054300683.1	MFS transporter	-
Psal161_RS17580	7793..7936	+	144	WP_155047030.1	hypothetical protein	-
Psal161_RS17585	7961..8392	+	432	WP_016211069.1	hypothetical protein	-
Psal161_RS17590	8376..9161	+	786	WP_032126922.1	queuosine precursor transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31951	31951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T123664 WP_027242955.1 NZ_CP039200:4014-4274 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T123664 NZ_CP039200:4014-4274 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTAATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13462.36 Da
• Isoelectric Point: 5.0200

>AT123664 WP_054300685.1 NZ_CP039200:4267-4620 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGGDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT123664 NZ_CP039200:4267-4620 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTGGTGATTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTGGA
AAAGTTAAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87