Detailed information of TA system TA123661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 93272..93842
• Replicon: plasmid unnamed1
• Accession: NZ_CP039196
• Organism: Piscirickettsia salmonis strain Psal-161

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Psal161_RS16200
• Protein ID: WP_036781349.1
• Coordinates: 93272..93598 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Psal161_RS16205
• Protein ID: WP_016212365.1
• Coordinates: 93600..93842 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal161_RS16165	88948..89325	+	378	Protein_121	DDE-type integrase/transposase/recombinase	-
Psal161_RS16170	89426..90205	+	780	WP_081377351.1	transposase	-
Psal161_RS16180	90394..90726	+	333	WP_032126739.1	transposase	-
Psal161_RS16185	90891..91268	+	378	WP_016212154.1	DDE-type integrase/transposase/recombinase	-
Psal161_RS16190	91575..91958	-	384	WP_016212152.1	hypothetical protein	-
Psal161_RS16195	92428..93156	-	729	WP_075273842.1	IS6 family transposase	-
Psal161_RS16200	93272..93598	-	327	WP_036781349.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Psal161_RS16205	93600..93842	-	243	WP_016212365.1	antidote-toxin recognition MazE family protein	Antitoxin
Psal161_RS16210	94047..94487	+	441	Protein_129	tyrosine-type recombinase/integrase	-
Psal161_RS16215	94555..95022	+	468	Protein_130	IS6-like element ISPsa2 family transposase	-
Psal161_RS16220	95196..95375	-	180	WP_155047017.1	hypothetical protein	-
Psal161_RS16225	95302..95571	+	270	Protein_132	DDE-type integrase/transposase/recombinase	-
Psal161_RS16230	95854..97488	+	1635	WP_075273844.1	protein kinase	-
Psal161_RS17965	97609..98340	+	732	WP_016212311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..158207	158207
	-	inside	IScluster/Tn	-	-	83940..99228	15288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11971.89 Da
• Isoelectric Point: 10.1733

>T123661 WP_036781349.1 NZ_CP039196:c93598-93272 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARGWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 327 bp

>T123661 NZ_CP039196:c93598-93272 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTGACTTCCAAGCCCTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGAGCAGTGTTGGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGGCTGGAATCATGCTGATAAGCTCGATAAAAAATCTCTTGAAAGAGTTCAGGGGCTAATTGATGTGATGTTGAA
AGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.09 Da
• Isoelectric Point: 4.4750

>AT123661 WP_016212365.1 NZ_CP039196:c93842-93600 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT123661 NZ_CP039196:c93842-93600 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGGAATAGCTTGGGGTTGTTGCTACCCTTGAGCATCGCACGTGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACTCATACGCAGACCCTTGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA