Detailed information of TA system TA123658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 14709..15279
• Replicon: plasmid unnamed2
• Accession: NZ_CP039192
• Organism: Piscirickettsia salmonis strain Psal-160

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Psal160_RS16535
• Protein ID: WP_054300674.1
• Coordinates: 14709..15035 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Psal160_RS16540
• Protein ID: WP_054300649.1
• Coordinates: 15037..15279 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal160_RS17565	10639..10815	-	177	WP_172673426.1	transposase	-
Psal160_RS17570	10809..11033	-	225	WP_196214208.1	hypothetical protein	-
Psal160_RS16520	11219..11518	+	300	WP_075273685.1	HU family DNA-binding protein	-
Psal160_RS16525	11631..13331	+	1701	WP_054300647.1	DUF4338 domain-containing protein	-
Psal160_RS16530	13357..14592	+	1236	WP_054300648.1	hypothetical protein	-
Psal160_RS16535	14709..15035	-	327	WP_054300674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Psal160_RS16540	15037..15279	-	243	WP_054300649.1	hypothetical protein	Antitoxin
Psal160_RS16545	15693..16013	-	321	WP_054300650.1	hypothetical protein	-
Psal160_RS16550	16034..16468	-	435	WP_054300651.1	hypothetical protein	-
Psal160_RS16555	16475..16882	-	408	WP_054300652.1	hypothetical protein	-
Psal160_RS16560	16981..17289	-	309	WP_081007087.1	helix-turn-helix transcriptional regulator	-
Psal160_RS16565	17339..17599	-	261	WP_054300654.1	hypothetical protein	-
Psal160_RS16570	17661..18218	-	558	WP_054300675.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82130	82130
	-	flank	IS/Tn	-	-	17661..18239	578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12091.96 Da
• Isoelectric Point: 9.9714

>T123658 WP_054300674.1 NZ_CP039192:c15035-14709 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARDWNHADKLDKKSLERVQGLIDVMFRG

Nucleotide

• Length: 327 bp

>T123658 NZ_CP039192:c15035-14709 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTAACTTCCAAGCCTTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGCGCAGTGCTAGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGACTGGAATCATGCTGATAAGCTCGATAAAAAATCACTTGAAAGAGTTCAAGGTTTAATTGACGTGATGTTTAG
AGGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.14 Da
• Isoelectric Point: 4.6318

>AT123658 WP_054300649.1 NZ_CP039192:c15279-15037 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTKTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT123658 NZ_CP039192:c15279-15037 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGTAATAGCTTGGGTTTATTATTGCCATTGAGCATTGCGCGCGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACGCATACGAAGACCCTAGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA