Detailed information of TA system TA123651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 5039..5609
• Replicon: plasmid unnamed2
• Accession: NZ_CP039183
• Organism: Piscirickettsia salmonis strain Psal-158

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Psal158_RS16405
• Protein ID: WP_054300674.1
• Coordinates: 5039..5365 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Psal158_RS16410
• Protein ID: WP_054300649.1
• Coordinates: 5367..5609 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal158_RS17525	969..1145	-	177	WP_172673426.1	transposase	-
Psal158_RS17530	1139..1363	-	225	WP_196214208.1	hypothetical protein	-
Psal158_RS16390	1549..1848	+	300	WP_075273685.1	HU family DNA-binding protein	-
Psal158_RS16395	1961..3661	+	1701	WP_054300647.1	DUF4338 domain-containing protein	-
Psal158_RS16400	3687..4922	+	1236	WP_054300648.1	hypothetical protein	-
Psal158_RS16405	5039..5365	-	327	WP_054300674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Psal158_RS16410	5367..5609	-	243	WP_054300649.1	hypothetical protein	Antitoxin
Psal158_RS16415	6023..6343	-	321	WP_054300650.1	hypothetical protein	-
Psal158_RS16420	6364..6798	-	435	WP_054300651.1	hypothetical protein	-
Psal158_RS16425	6805..7212	-	408	WP_054300652.1	hypothetical protein	-
Psal158_RS16430	7311..7619	-	309	WP_081007087.1	helix-turn-helix transcriptional regulator	-
Psal158_RS16435	7669..7929	-	261	WP_054300654.1	hypothetical protein	-
Psal158_RS16440	7991..8548	-	558	WP_054300675.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82130	82130
	-	flank	IS/Tn	-	-	7991..8569	578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12091.96 Da
• Isoelectric Point: 9.9714

>T123651 WP_054300674.1 NZ_CP039183:c5365-5039 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARDWNHADKLDKKSLERVQGLIDVMFRG

Nucleotide

• Length: 327 bp

>T123651 NZ_CP039183:c5365-5039 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTAACTTCCAAGCCTTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGCGCAGTGCTAGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGACTGGAATCATGCTGATAAGCTCGATAAAAAATCACTTGAAAGAGTTCAAGGTTTAATTGACGTGATGTTTAG
AGGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.14 Da
• Isoelectric Point: 4.6318

>AT123651 WP_054300649.1 NZ_CP039183:c5609-5367 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTKTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT123651 NZ_CP039183:c5609-5367 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGTAATAGCTTGGGTTTATTATTGCCATTGAGCATTGCGCGCGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACGCATACGAAGACCCTAGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA