Detailed information of TA system TA123628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2078517..2079046
• Replicon: chromosome
• Accession: NZ_CP039167
• Organism: Staphylococcus aureus strain R50

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E1952_RS10705
• Protein ID: WP_000621175.1
• Coordinates: 2078517..2078879 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E1952_RS10710
• Protein ID: WP_000948331.1
• Coordinates: 2078876..2079046 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1952_RS10685	2075496..2076266	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
E1952_RS10690	2076241..2076720	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
E1952_RS10695	2076722..2077048	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E1952_RS10700	2077167..2078168	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E1952_RS10705	2078517..2078879	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E1952_RS10710	2078876..2079046	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E1952_RS10715	2079131..2080279	-	1149	WP_001281154.1	alanine racemase	-
E1952_RS10720	2080345..2080704	-	360	WP_000581197.1	holo-ACP synthase	-
E1952_RS10725	2080708..2081199	-	492	WP_001205912.1	PH domain-containing protein	-
E1952_RS10730	2081186..2082769	-	1584	WP_001294620.1	PH domain-containing protein	-
E1952_RS10735	2082762..2083241	-	480	WP_001287079.1	hypothetical protein	-
E1952_RS10740	2083450..2084010	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T123628 WP_000621175.1 NZ_CP039167:c2078879-2078517 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T123628 NZ_CP039167:c2078879-2078517 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT123628 WP_000948331.1 NZ_CP039167:c2079046-2078876 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT123628 NZ_CP039167:c2079046-2078876 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1