Detailed information of TA system TA123607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2015211..2015740
• Replicon: chromosome
• Accession: NZ_CP039162
• Organism: Staphylococcus aureus strain Lr12

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E1950_RS10230
• Protein ID: WP_000621175.1
• Coordinates: 2015211..2015573 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E1950_RS10235
• Protein ID: WP_000948331.1
• Coordinates: 2015570..2015740 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1950_RS10210	2012189..2012959	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
E1950_RS10215	2012934..2013413	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
E1950_RS10220	2013415..2013741	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E1950_RS10225	2013860..2014861	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E1950_RS10230	2015211..2015573	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E1950_RS10235	2015570..2015740	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E1950_RS10240	2015825..2016973	-	1149	WP_001281154.1	alanine racemase	-
E1950_RS10245	2017039..2017398	-	360	WP_000581197.1	holo-ACP synthase	-
E1950_RS10250	2017402..2017893	-	492	WP_001205912.1	PH domain-containing protein	-
E1950_RS10255	2017880..2019463	-	1584	WP_001294626.1	PH domain-containing protein	-
E1950_RS10260	2019456..2019935	-	480	WP_001287087.1	hypothetical protein	-
E1950_RS10265	2020143..2020703	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T123607 WP_000621175.1 NZ_CP039162:c2015573-2015211 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T123607 NZ_CP039162:c2015573-2015211 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT123607 WP_000948331.1 NZ_CP039162:c2015740-2015570 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT123607 NZ_CP039162:c2015740-2015570 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1