Detailed information of TA system TA123592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2041962..2042491
• Replicon: chromosome
• Accession: NZ_CP039160
• Organism: Staphylococcus aureus strain Lr6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E1949_RS10420
• Protein ID: WP_000621175.1
• Coordinates: 2041962..2042324 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E1949_RS10425
• Protein ID: WP_000948331.1
• Coordinates: 2042321..2042491 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1949_RS10400	2038941..2039711	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
E1949_RS10405	2039686..2040165	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
E1949_RS10410	2040167..2040493	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E1949_RS10415	2040612..2041613	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E1949_RS10420	2041962..2042324	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E1949_RS10425	2042321..2042491	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E1949_RS10430	2042576..2043724	-	1149	WP_001281154.1	alanine racemase	-
E1949_RS10435	2043790..2044149	-	360	WP_000581197.1	holo-ACP synthase	-
E1949_RS10440	2044153..2044644	-	492	WP_001205912.1	PH domain-containing protein	-
E1949_RS10445	2044631..2046214	-	1584	WP_001294620.1	PH domain-containing protein	-
E1949_RS10450	2046207..2046686	-	480	WP_001287079.1	hypothetical protein	-
E1949_RS10455	2046895..2047455	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T123592 WP_000621175.1 NZ_CP039160:c2042324-2041962 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T123592 NZ_CP039160:c2042324-2041962 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT123592 WP_000948331.1 NZ_CP039160:c2042491-2042321 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT123592 NZ_CP039160:c2042491-2042321 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1