Detailed information of TA system TA123558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2609..3186
• Replicon: plasmid p10FS3-1-12
• Accession: NZ_CP039155
• Organism: Acinetobacter sp. 10FS3-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: E5Y90_RS17340
• Protein ID: WP_004897309.1
• Coordinates: 2609..2857 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E5Y90_RS17345
• Protein ID: WP_174660750.1
• Coordinates: 2854..3186 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Y90_RS17325	1..5881	-	5881	Protein_0	type II toxin-antitoxin system RelE/ParE family toxin	-
E5Y90_RS17330	1010..1549	-	540	WP_174660748.1	helix-turn-helix domain-containing protein	-
E5Y90_RS17335	2193..2453	-	261	WP_174660749.1	hypothetical protein	-
E5Y90_RS17340	2609..2857	+	249	WP_004897309.1	type II toxin-antitoxin system HicA family toxin	Toxin
E5Y90_RS17345	2854..3186	+	333	WP_174660750.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E5Y90_RS17350	3408..4437	+	1030	Protein_5	transposase	-
E5Y90_RS17355	4558..4794	+	237	WP_174660751.1	DUF1090 family protein	-
E5Y90_RS17360	5150..5443	-	294	WP_174660752.1	hypothetical protein	-
E5Y90_RS17365	5453..5746	-	294	WP_174660753.1	HigA family addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..5881	5881
	-	flank	IS/Tn	-	-	3408..4226	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-78)

Antitoxin

• Uncharacterised protein family HicB (54-101)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9501.14 Da
• Isoelectric Point: 10.3185

>T123558 WP_004897309.1 NZ_CP039155:2609-2857 [Acinetobacter sp. 10FS3-1]
MGKTEKLLEKLGNSKNTFPYKDLVVLLSQLGYEKFEMAGSRVRFFNEKTEHMILLHRPHPENEIKGGALKAVKQALKQER
FL

Nucleotide

• Length: 249 bp

>T123558 NZ_CP039155:2609-2857 [Acinetobacter sp. 10FS3-1]
ATGGGTAAAACGGAAAAGTTATTGGAAAAACTTGGAAATTCAAAAAATACATTTCCATATAAAGATTTAGTCGTTTTACT
AAGCCAACTCGGTTATGAAAAGTTTGAAATGGCAGGCTCTCGTGTTCGATTTTTTAATGAAAAAACTGAACATATGATTT
TACTGCATAGACCACACCCTGAGAATGAAATCAAAGGTGGTGCATTGAAAGCTGTAAAACAAGCACTCAAGCAGGAGAGA
TTTTTATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12394.20 Da
• Isoelectric Point: 5.1972

>AT123558 WP_174660750.1 NZ_CP039155:2854-3186 [Acinetobacter sp. 10FS3-1]
MSYLKYKGYLGTIEPDLETGELFGKVAFIRDLITYEAETLKALEQAFQESVDGYLESCIELNKKPDQPFKGTFNVRISPD
LHRKAVLASSNSLNKFVSDAIQEKLMRLGA

Nucleotide

• Length: 333 bp

>AT123558 NZ_CP039155:2854-3186 [Acinetobacter sp. 10FS3-1]
ATGAGTTATCTAAAATATAAAGGTTATCTTGGAACAATTGAACCTGACCTAGAAACAGGTGAGTTGTTTGGAAAAGTCGC
TTTTATCCGTGATTTGATCACTTATGAAGCCGAAACATTAAAAGCATTAGAACAAGCCTTTCAAGAATCTGTAGATGGCT
ATTTAGAATCATGTATCGAACTAAATAAAAAGCCTGATCAACCCTTTAAAGGTACGTTTAATGTGCGTATCAGTCCTGAT
CTACACCGTAAAGCTGTTTTAGCATCAAGTAATTCATTGAATAAGTTTGTAAGCGATGCAATTCAAGAAAAGTTAATGCG
TTTAGGTGCTTAA