Detailed information of TA system TA123556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 8298..8875
• Replicon: plasmid p10FS3-1-7
• Accession: NZ_CP039150
• Organism: Acinetobacter sp. 10FS3-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: N8ZPI0
• Locus tag: E5Y90_RS17070
• Protein ID: WP_004812233.1
• Coordinates: 8627..8875 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: N8ZW11
• Locus tag: E5Y90_RS17065
• Protein ID: WP_004812232.1
• Coordinates: 8298..8630 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Y90_RS17040	3358..4641	+	1284	WP_174660714.1	MobA/MobL family protein	-
E5Y90_RS17045	4625..4858	-	234	WP_174660715.1	hypothetical protein	-
E5Y90_RS17050	5308..5709	+	402	WP_174660716.1	PH domain-containing protein	-
E5Y90_RS17055	6574..7461	-	888	WP_174660717.1	LysR family transcriptional regulator ArgP	-
E5Y90_RS17060	7559..8155	+	597	WP_174660718.1	LysE/ArgO family amino acid transporter	-
E5Y90_RS17065	8298..8630	-	333	WP_004812232.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E5Y90_RS17070	8627..8875	-	249	WP_004812233.1	type II toxin-antitoxin system HicA family toxin	Toxin
E5Y90_RS17075	9016..9540	-	525	WP_131275023.1	inorganic diphosphatase	-
E5Y90_RS17080	9567..9947	-	381	WP_005225409.1	fluoride efflux transporter CrcB	-
E5Y90_RS17085	10330..10539	+	210	WP_004282305.1	hypothetical protein	-
E5Y90_RS17090	10791..12245	+	1455	WP_151819645.1	SulP family inorganic anion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12304	12304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-78)

Antitoxin

• Uncharacterised protein family HicB (54-101)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9436.02 Da
• Isoelectric Point: 10.1337

>T123556 WP_004812233.1 NZ_CP039150:c8875-8627 [Acinetobacter sp. 10FS3-1]
MGKTEKLLEKFGNSKNTFPYKDLVVLLSQLGYEKFEMAGSRVRFFNEKTEHMILLHRPHPENEIKGGALKAVKQALKQEG
FL

Nucleotide

• Length: 249 bp

>T123556 NZ_CP039150:c8875-8627 [Acinetobacter sp. 10FS3-1]
ATGGGTAAAACGGAAAAGTTATTGGAAAAATTTGGAAATTCAAAAAATACGTTTCCATATAAAGATTTAGTCGTTTTACT
GAGCCAGCTCGGCTATGAAAAATTTGAAATGGCAGGCTCTCGTGTTCGGTTTTTTAATGAAAAAACTGAACATATGATTT
TACTGCATAGACCACACCCTGAAAATGAAATCAAAGGTGGGGCATTGAAAGCTGTAAAACAGGCACTCAAGCAGGAGGGA
TTTTTATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12224.97 Da
• Isoelectric Point: 4.8951

>AT123556 WP_004812232.1 NZ_CP039150:c8630-8298 [Acinetobacter sp. 10FS3-1]
MSYLKYKGYLGTIEPDLETGELFGKLAFIRDLITYEAETLKALEKAFQESVDGYLESCAELGKSPDQPFKGTFNVRISPE
LHRKAVLASSNSLNAFVSDAIQEKLMRLGA

Nucleotide

• Length: 333 bp

>AT123556 NZ_CP039150:c8630-8298 [Acinetobacter sp. 10FS3-1]
ATGAGCTATTTAAAATACAAAGGTTATCTCGGAACAATTGAGCCTGATCTTGAAACAGGTGAGTTATTTGGAAAACTTGC
TTTTATTCGTGATCTTATTACCTATGAAGCGGAAACTTTAAAAGCCCTTGAGAAAGCTTTTCAGGAATCTGTAGACGGCT
ATTTAGAATCATGTGCTGAACTAGGAAAATCTCCTGATCAACCATTCAAAGGCACTTTTAATGTCCGTATTAGCCCTGAA
TTACACCGTAAGGCGGTTTTAGCATCAAGTAATTCTCTAAATGCCTTTGTCAGTGATGCGATTCAAGAAAAGTTAATGCG
TTTAGGTGCGTAA

Structures

Toxin

• AlphaFold DB: N8ZPI0

Antitoxin

• AlphaFold DB: N8ZW11