Detailed information of TA system TA123555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4652..5237
• Replicon: plasmid p10FS3-1-6
• Accession: NZ_CP039149
• Organism: Acinetobacter sp. 10FS3-1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: E5Y90_RS16980
• Protein ID: WP_151837470.1
• Coordinates: 4652..4939 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: E5Y90_RS16985
• Protein ID: WP_174660705.1
• Coordinates: 4926..5237 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Y90_RS16955	1..924	+	924	WP_005021644.1	replication initiation protein RepM	-
E5Y90_RS16960	1009..1542	+	534	WP_033107830.1	plasmid replication DNA-binding protein	-
E5Y90_RS16965	1807..2349	-	543	WP_004637127.1	cysteine hydrolase	-
E5Y90_RS16970	2433..3317	-	885	WP_005176778.1	MBL fold metallo-hydrolase	-
E5Y90_RS16975	3450..4421	+	972	WP_174660704.1	helix-turn-helix domain-containing protein	-
E5Y90_RS16980	4652..4939	+	288	WP_151837470.1	BrnT family toxin	Toxin
E5Y90_RS16985	4926..5237	+	312	WP_174660705.1	BrnA antitoxin family protein	Antitoxin
E5Y90_RS16990	5570..7087	+	1518	WP_174660706.1	catalase	-
E5Y90_RS16995	7265..7753	+	489	WP_085064972.1	ankyrin repeat domain-containing protein	-
E5Y90_RS17000	7839..9359	-	1521	WP_043041729.1	catalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..13219	13219

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11248.59 Da
• Isoelectric Point: 5.1978

>T123555 WP_151837470.1 NZ_CP039149:4652-4939 [Acinetobacter sp. 10FS3-1]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T123555 NZ_CP039149:4652-4939 [Acinetobacter sp. 10FS3-1]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCACGATGTCTCTTTCGAAACGGCAAG
CCTTGTCTTTGATGATCCACTGAGGATCTCAATTCAGGACAGACATACCGATGGCGAAGAACGTTGGCAGACCATTGGAA
AAGTGAAAGGCGTACTAATGCTCTTAGTAGCTCATACCATCTTTGATGAAGATGACTGTGAAATCATAAGAATCATTAGT
GCAAGACAGGTCACTAAAGCGGAGCGCAATCAATATGAGCACGGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 12018.73 Da
• Isoelectric Point: 9.9265

>AT123555 WP_174660705.1 NZ_CP039149:4926-5237 [Acinetobacter sp. 10FS3-1]
MSTVRYSRKELNEKFSEKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAIRHGQFFRPVKQQTSVRLDADVLAWLKAQ
GKGYQTRMNKILREAMLKDLKNH

Nucleotide

• Length: 312 bp

>AT123555 NZ_CP039149:4926-5237 [Acinetobacter sp. 10FS3-1]
ATGAGCACGGTTAGATACTCACGCAAAGAACTAAATGAAAAATTTAGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATGCCTGAAATTACCGACTGGAGTAATGCTATTCGTC
ATGGGCAATTTTTTCGTCCAGTGAAGCAACAAACGTCAGTTCGTCTTGATGCGGATGTATTAGCATGGTTAAAAGCACAA
GGAAAAGGTTATCAAACACGTATGAATAAAATTCTGCGTGAAGCAATGCTAAAAGATTTAAAAAATCATTAA