Detailed information of TA system TA123554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 8056..8644
• Replicon: plasmid p10FS3-1-5
• Accession: NZ_CP039148
• Organism: Acinetobacter sp. 10FS3-1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: E5Y90_RS16910
• Protein ID: WP_174660697.1
• Coordinates: 8056..8343 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A6N0LIV1
• Locus tag: E5Y90_RS16915
• Protein ID: WP_000108900.1
• Coordinates: 8330..8644 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Y90_RS16890	3188..4120	-	933	WP_174659137.1	IS5 family transposase	-
E5Y90_RS16895	4627..5238	-	612	WP_004889724.1	recombinase family protein	-
E5Y90_RS16900	5423..5812	-	390	WP_046206304.1	tautomerase family protein	-
E5Y90_RS16905	5973..7385	-	1413	WP_046206303.1	PLP-dependent aminotransferase family protein	-
E5Y90_RS16910	8056..8343	+	288	WP_174660697.1	BrnT family toxin	Toxin
E5Y90_RS16915	8330..8644	+	315	WP_000108900.1	BrnA antitoxin family protein	Antitoxin
E5Y90_RS16920	8835..9395	+	561	WP_174660698.1	hypothetical protein	-
E5Y90_RS16925	9712..10266	-	555	WP_174660699.1	hypothetical protein	-
E5Y90_RS16930	10474..10713	+	240	WP_174660700.1	hypothetical protein	-
E5Y90_RS16935	10878..11537	-	660	WP_174660701.1	protein phosphatase 2C domain-containing protein	-
E5Y90_RS16940	11595..12902	-	1308	WP_174660702.1	serine/threonine protein kinase	-
E5Y90_RS16945	13025..13390	-	366	WP_125275264.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..16045	16045
	-	flank	IS/Tn	-	-	3188..4120	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11277.66 Da
• Isoelectric Point: 5.6212

>T123554 WP_174660697.1 NZ_CP039148:8056-8343 [Acinetobacter sp. 10FS3-1]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDGFEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T123554 NZ_CP039148:8056-8343 [Acinetobacter sp. 10FS3-1]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACGGCAAG
CCTTGTTTTTGAAGATCCTCTGAGGATCTCAATTCAGGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
GAGTAAAAGGCGTACTAATGCTATTAGTAGCTCACACCATCTTCGATGAAGATGGCTTTGAAATCATACGAATCATTAGT
GCAAGACAAGTCACTAAGGCGGAGCGAGATAAATATGAGCACGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12236.83 Da
• Isoelectric Point: 8.4842

>AT123554 WP_000108900.1 NZ_CP039148:8330-8644 [Acinetobacter sp. 10FS3-1]
MSTVRYSRKELNEKFSEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT123554 NZ_CP039148:8330-8644 [Acinetobacter sp. 10FS3-1]
ATGAGCACGGTTAGATACTCACGCAAAGAACTAAATGAAAAATTTAGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGGACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGTAATGCTGTACGCC
ATAATCAATTCTATCGCCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGTAAAGGCTATCAAACACGAATGAATGAAATTTTACGAGATGCTATGCTCAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6N0LIV1