Detailed information of TA system TA123550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 300494..301636
• Replicon: plasmid p10FS3-1-1
• Accession: NZ_CP039144
• Organism: Acinetobacter sp. 10FS3-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: E5Y90_RS15485
• Protein ID: WP_163146385.1
• Coordinates: 300872..301636 (+)
• Length: 255 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: E5Y90_RS15480
• Protein ID: WP_163146384.1
• Coordinates: 300494..300862 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Y90_RS15455	296046..297065	+	1020	WP_163146380.1	hypothetical protein	-
E5Y90_RS15460	297132..297551	+	420	WP_163146381.1	acyl-CoA thioesterase	-
E5Y90_RS15465	297584..298516	+	933	WP_163146382.1	DMT family transporter	-
E5Y90_RS15470	298509..299246	+	738	WP_163146383.1	acetoacetyl-CoA reductase	-
E5Y90_RS15475	299417..300348	+	932	Protein_292	IS5 family transposase	-
E5Y90_RS15480	300494..300862	+	369	WP_163146384.1	XRE family transcriptional regulator	Antitoxin
E5Y90_RS15485	300872..301636	+	765	WP_163146385.1	RES family NAD+ phosphorylase	Toxin
E5Y90_RS15490	301693..302076	-	384	WP_163146386.1	hypothetical protein	-
E5Y90_RS15495	302200..303102	+	903	WP_163146387.1	hypothetical protein	-
E5Y90_RS15500	303177..303314	-	138	WP_163146388.1	hypothetical protein	-
E5Y90_RS15505	303333..304007	-	675	WP_163146389.1	hypothetical protein	-
E5Y90_RS15510	304135..304728	+	594	WP_163146390.1	hypothetical protein	-
E5Y90_RS15515	304768..306198	+	1431	WP_163146391.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..395494	395494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (71-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-55)

Sequences

Toxin

Protein

• Length: 255 a.a.
• Molecular weight: 28860.46 Da
• Isoelectric Point: 4.8115

>T123550 WP_163146385.1 NZ_CP039144:300872-301636 [Acinetobacter sp. 10FS3-1]
MKATDSIWKACRGEEQIVPVSGVLFRMVKKLEQIATWQIVENLGEQAELEDLIEEKISAVSSLPKEDELHILLKAPFQYP
PLEWGSRFGRTHEPSIFYGGCSVDATLAELAYYRFVYLQNIDTNSGESMQSQHLMFSVGYQSEKGIRLQDPPFDQHHDLI
AHPSNYSRAQQLGAEMRVAEVEVFEYPSSRAADHAPCVGIFTPGNFTQNYPLSQEEWLSELTRDTVTFKSMGSRLVKTYS
ISDFFHNGEFPRPA

Nucleotide

• Length: 765 bp

>T123550 NZ_CP039144:300872-301636 [Acinetobacter sp. 10FS3-1]
ATGAAAGCTACGGACAGCATTTGGAAAGCATGTCGCGGTGAAGAGCAGATAGTTCCAGTATCAGGTGTACTGTTTCGCAT
GGTTAAAAAACTGGAACAGATCGCCACCTGGCAAATTGTGGAAAATCTGGGTGAACAGGCTGAACTTGAAGATTTGATCG
AAGAAAAGATATCGGCAGTATCAAGCCTACCTAAAGAAGATGAACTACACATTCTGTTAAAAGCACCGTTTCAATATCCA
CCGTTAGAATGGGGTTCTCGATTTGGGCGTACCCATGAGCCGAGTATTTTTTATGGTGGGTGTAGTGTTGACGCGACATT
GGCTGAATTGGCTTACTATCGTTTTGTTTATTTGCAAAATATAGATACTAATTCTGGGGAATCAATGCAGTCACAACATT
TGATGTTCTCAGTTGGCTATCAGTCAGAGAAGGGTATTCGATTACAGGACCCGCCATTTGATCAGCACCATGATTTGATT
GCGCATCCATCTAATTACAGTCGTGCTCAACAATTAGGTGCTGAAATGCGTGTGGCAGAAGTTGAAGTGTTTGAATACCC
ATCTTCACGAGCTGCTGATCATGCGCCTTGTGTTGGTATTTTCACACCAGGTAACTTCACTCAAAACTATCCTCTGTCAC
AGGAAGAGTGGCTAAGTGAGTTGACTAGGGATACCGTGACATTCAAATCAATGGGTAGCCGTCTTGTTAAGACATATTCA
ATCAGTGATTTTTTTCACAACGGCGAATTTCCAAGACCGGCTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13318.50 Da
• Isoelectric Point: 10.2156

>AT123550 WP_163146384.1 NZ_CP039144:300494-300862 [Acinetobacter sp. 10FS3-1]
MSDVVERPKVDRKAVLSKALLNAADQLKINQADLAEVIGVHRTAISKLKKKPDLDPDSKAGELAVLFIRLYRGVYALNGG
NQDWMLRFLNSPNKATGGVPAQQIKTVQGLVHVVQYLDAMPG

Nucleotide

• Length: 369 bp

>AT123550 NZ_CP039144:300494-300862 [Acinetobacter sp. 10FS3-1]
ATGTCTGATGTTGTTGAAAGACCAAAAGTAGATCGGAAAGCTGTACTCTCCAAAGCCTTATTAAATGCAGCCGATCAGCT
CAAGATCAATCAAGCTGATCTTGCCGAGGTGATTGGTGTTCACCGTACTGCAATTAGCAAATTGAAAAAGAAACCTGATC
TGGACCCGGATTCTAAAGCCGGAGAATTAGCAGTGTTGTTTATTCGCTTATATCGTGGTGTGTACGCACTCAACGGCGGT
AATCAGGATTGGATGCTTCGCTTCCTTAATTCACCTAACAAAGCAACTGGCGGTGTGCCGGCGCAGCAAATCAAGACCGT
TCAAGGTCTAGTGCATGTAGTTCAGTATTTAGATGCAATGCCAGGATAA