123547

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5234054..5234685 Replicon chromosome
Accession NZ_CP039127
Organism Pseudomonas sp. A2

Toxin (Protein)


Gene name hicA Uniprot ID A0A0C1KJT5
Locus tag E5221_RS24270 Protein ID WP_003258415.1
Coordinates 5234458..5234685 (-) Length 76 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V9X203
Locus tag E5221_RS24265 Protein ID WP_003258416.1
Coordinates 5234054..5234461 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5221_RS24245 (E5221_25130) 5230708..5232039 + 1332 WP_247846560.1 phosphatase PAP2/dual specificity phosphatase family protein -
E5221_RS24250 (E5221_25135) 5232036..5232467 + 432 WP_247846561.1 hypothetical protein -
E5221_RS24255 (E5221_25140) 5232478..5233101 + 624 WP_247846562.1 lysophospholipid acyltransferase family protein -
E5221_RS24260 (E5221_25145) 5233103..5234035 + 933 WP_039613060.1 phosphatidate cytidylyltransferase -
E5221_RS24265 (E5221_25150) 5234054..5234461 - 408 WP_003258416.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E5221_RS24270 (E5221_25155) 5234458..5234685 - 228 WP_003258415.1 type II toxin-antitoxin system HicA family toxin Toxin
E5221_RS24275 (E5221_25160) 5234882..5235157 - 276 WP_003258414.1 metal/formaldehyde-sensitive transcriptional repressor -
E5221_RS24280 (E5221_25165) 5235228..5236175 + 948 WP_247846563.1 CDF family Co(II)/Ni(II) efflux transporter DmeF -
E5221_RS24285 (E5221_25170) 5236272..5236616 + 345 WP_003258412.1 cupin domain-containing protein -
E5221_RS24290 (E5221_25175) 5236924..5237412 + 489 WP_247846564.1 type VI secretion system tube protein Hcp -
E5221_RS24295 (E5221_25180) 5237423..5238400 + 978 WP_247846565.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 76 a.a.        Molecular weight: 8270.38 Da        Isoelectric Point: 10.9929

>T123547 WP_003258415.1 NZ_CP039127:c5234685-5234458 [Pseudomonas sp. A2]
MRSREVIQLIEADGWYQVDVKGSHYQFRHPTKRGRVTVPHPKSELPKGTVRSILKQAGLIQANGTASTSINGGSQ

Download         Length: 228 bp

>T123547 NZ_CP039127:c5234685-5234458 [Pseudomonas sp. A2]
ATGCGCAGTCGTGAAGTTATCCAGCTTATTGAAGCCGACGGCTGGTACCAGGTTGATGTGAAAGGTAGTCATTACCAGTT
CAGGCATCCGACAAAAAGGGGGCGAGTCACGGTTCCTCATCCCAAGAGTGAGCTCCCCAAGGGCACAGTACGCAGCATCT
TGAAGCAGGCTGGCTTGATACAAGCCAACGGCACTGCCAGCACGAGCATCAACGGAGGTAGTCAATGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14673.52 Da        Isoelectric Point: 4.7475

>AT123547 WP_003258416.1 NZ_CP039127:c5234461-5234054 [Pseudomonas sp. A2]
MKFPVVLHKDADSDYGVTVPDVPGCFSAGATVAKALENVQEALALHFEGLVADNEALPQAQEVDVHVANPDYEGGVWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELALSH

Download         Length: 408 bp

>AT123547 NZ_CP039127:c5234461-5234054 [Pseudomonas sp. A2]
ATGAAATTCCCGGTCGTATTGCACAAAGACGCCGACTCGGACTACGGCGTGACCGTACCTGATGTGCCCGGTTGCTTCAG
CGCCGGTGCCACTGTTGCAAAAGCACTGGAGAATGTTCAGGAAGCGCTGGCACTACATTTTGAAGGGTTGGTCGCAGATA
ACGAGGCATTGCCGCAGGCACAGGAAGTCGACGTCCATGTGGCAAACCCTGACTATGAAGGCGGGGTCTGGGCCGTTGTG
GATTTTGATGTCACCCCGTACCTGGGTAAGGCGGTTCGCTTCAATGCAACGCTGCCGGAAAACCTGCTTCAACGAATCGA
CGAAAAAGTGAAGCGTGATCATCGCTATGCGTCCCGCTCCGGTTTTCTGGCTTCGGCTGCACTGCGAGAGTTGGCCCTTT
CGCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C1KJT5

Antitoxin

Source ID Structure
AlphaFold DB V9X203

References