Detailed information of TA system TA123546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3752041..3752924
• Replicon: chromosome
• Accession: NZ_CP039127
• Organism: Pseudomonas sp. A2

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A0C1HTG7
• Locus tag: E5221_RS17300
• Protein ID: WP_015270851.1
• Coordinates: 3752041..3752478 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: E5221_RS17305
• Protein ID: WP_182329420.1
• Coordinates: 3752475..3752924 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5221_RS17285 (E5221_17890)	3748411..3749640	-	1230	WP_013973159.1	acyl-CoA dehydrogenase	-
E5221_RS17290 (E5221_17895)	3749781..3750707	+	927	WP_013973160.1	LysR family transcriptional regulator	-
E5221_RS17295 (E5221_17900)	3750879..3752033	+	1155	WP_054572084.1	aminotransferase class V-fold PLP-dependent enzyme	-
E5221_RS17300 (E5221_17905)	3752041..3752478	-	438	WP_015270851.1	RES family NAD+ phosphorylase	Toxin
E5221_RS17305 (E5221_17910)	3752475..3752924	-	450	WP_182329420.1	DUF2384 domain-containing protein	Antitoxin
E5221_RS17310 (E5221_17915)	3753023..3753676	-	654	WP_182329419.1	oxygen-insensitive NAD(P)H nitroreductase	-
E5221_RS17315 (E5221_17920)	3753781..3754704	+	924	WP_015270853.1	LysR family transcriptional regulator	-
E5221_RS17320 (E5221_17925)	3754752..3755582	+	831	WP_247846068.1	AraC family transcriptional regulator	-
E5221_RS17325 (E5221_17930)	3755633..3756217	+	585	WP_061551750.1	LysE family translocator	-
E5221_RS17330 (E5221_17935)	3756276..3757493	-	1218	WP_247846069.1	sugar transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-137)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15969.19 Da
• Isoelectric Point: 5.1199

>T123546 WP_015270851.1 NZ_CP039127:c3752478-3752041 [Pseudomonas sp. A2]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPAGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSSQTELTRGLGNRFLDECSALLLPVPSAIMPSTTNYLFNPRHPQANSARLEVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T123546 NZ_CP039127:c3752478-3752041 [Pseudomonas sp. A2]
GTGATTCTGTGGCGTATCAGCGCCTATGCAGACTTGAGCGGAACGGGCGGCTTGCGTGTCAGCGGCCGCTGGCACCAGGC
GGGCCGGCCAGTGGTGTATGCCGCCACCAGCCCGGCCGGGGCGATGCTGGAGGTGCTGGTGCACCTGGAGATCGACCCTG
AGGATTTCCCCACCACCATGCGGCTGCTGCGTATCGAGTTGCCCGACACCGTTTCGCAAGCGCAGTTGCCCGCCTTGCAG
CCTGGCTGGTCGAGCCAGACCGAACTGACCCGGGGCCTGGGCAACCGTTTTCTGGATGAGTGTTCGGCGTTGCTGCTGCC
GGTGCCGAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCGCGCCATCCGCAGGCAAACAGCGCCAGGCTCG
AGGTCGAGGACTTCACCCCGGACAGCCGGCTGTTTTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17034.62 Da
• Isoelectric Point: 6.3405

>AT123546 WP_182329420.1 NZ_CP039127:c3752924-3752475 [Pseudomonas sp. A2]
MLAEVLRDNGYHEYRARLHALLEIPELASDFEIHTRITHGFAATWLVKLTELGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEASKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Nucleotide

• Length: 450 bp

>AT123546 NZ_CP039127:c3752924-3752475 [Pseudomonas sp. A2]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCATGCACTGCTGGAAATCCCCGAACT
CGCCAGTGACTTCGAAATCCACACCCGCATCACCCATGGCTTCGCTGCCACCTGGCTGGTGAAACTGACCGAACTCGGCG
TGTTCACCCCTGTGGAGCGTGACCAGATCATTCCCTTGCGCACGCTGAAAACCCGCATCGAACGTGACCAGCCGCTGACC
GTGGAAGAAAGCGACCGGCTGTTCCGCTCGGCCCATATCACCGCCATGGCCGAAGCCGTGTTCGGTGAAGCGAGCAAGGC
AAAACGCTGGCTGTCCAAGCCCAAGGAGCGCTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAGGGCACCA
CCCAGGTCGAAGAGATGTTGCTGCAGATCGCCGAGGGCTTCGGCCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0C1HTG7