Detailed information of TA system TA123545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2481038..2481554
• Replicon: chromosome
• Accession: NZ_CP039127
• Organism: Pseudomonas sp. A2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8G1E7
• Locus tag: E5221_RS11595
• Protein ID: WP_003259987.1
• Coordinates: 2481273..2481554 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8G1E6
• Locus tag: E5221_RS11590
• Protein ID: WP_013972043.1
• Coordinates: 2481038..2481283 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5221_RS11570 (E5221_11955)	2476428..2476970	-	543	WP_247847460.1	WYL domain-containing protein	-
E5221_RS11575 (E5221_11960)	2477078..2478064	-	987	WP_061550931.1	zinc-binding dehydrogenase	-
E5221_RS11580 (E5221_11965)	2478464..2479657	+	1194	WP_247847462.1	MFS transporter	-
E5221_RS11585 (E5221_11970)	2479688..2480794	+	1107	WP_247847463.1	alkene reductase	-
E5221_RS11590 (E5221_11975)	2481038..2481283	+	246	WP_013972043.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E5221_RS11595 (E5221_11980)	2481273..2481554	+	282	WP_003259987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5221_RS11600 (E5221_11985)	2481594..2481989	-	396	WP_023662326.1	hypothetical protein	-
E5221_RS11605 (E5221_11990)	2482078..2482986	-	909	WP_013972045.1	LysR family transcriptional regulator	-
E5221_RS11610 (E5221_12000)	2483278..2484582	+	1305	WP_247847465.1	MFS transporter	-
E5221_RS11615 (E5221_12005)	2484613..2485854	+	1242	WP_015269941.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10909.78 Da
• Isoelectric Point: 10.7594

>T123545 WP_003259987.1 NZ_CP039127:2481273-2481554 [Pseudomonas sp. A2]
MTYKLEFLPSALKEWGKLGHTVREQIKKKLAERLQSPKVQADALRDLPNHYKIKLKASGYRLVYRVEDERIVVVVVSVGK
RERSEVYRSAQKR

Nucleotide

• Length: 282 bp

>T123545 NZ_CP039127:2481273-2481554 [Pseudomonas sp. A2]
ATGACCTATAAACTCGAATTCCTGCCTTCGGCACTGAAAGAGTGGGGCAAGCTAGGCCACACCGTGCGCGAACAGATCAA
GAAAAAACTCGCCGAGCGCCTGCAGTCACCCAAGGTACAGGCCGACGCGCTGCGTGACCTGCCGAACCACTACAAAATCA
AGCTCAAGGCCTCTGGATATCGCCTCGTCTATCGAGTCGAGGACGAGCGTATCGTTGTAGTGGTGGTATCGGTTGGCAAG
CGCGAGCGTAGCGAGGTGTATCGATCCGCTCAGAAGCGGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8779.21 Da
• Isoelectric Point: 4.3611

>AT123545 WP_013972043.1 NZ_CP039127:2481038-2481283 [Pseudomonas sp. A2]
MQNIFADVAVSVSELKKNPSAVMTGAAGMPVAVLNHNRVMGYMVPAELYEAMMERLEDLELSELAKSRLGEKGVPVSLDD
L

Nucleotide

• Length: 246 bp

>AT123545 NZ_CP039127:2481038-2481283 [Pseudomonas sp. A2]
ATGCAAAACATATTCGCTGACGTGGCCGTAAGCGTGTCGGAACTGAAAAAGAACCCATCCGCGGTGATGACCGGCGCTGC
GGGTATGCCCGTCGCTGTGCTCAATCACAACCGCGTGATGGGTTACATGGTGCCGGCCGAACTGTACGAGGCCATGATGG
AACGCTTGGAAGATCTCGAATTGAGCGAACTGGCCAAGTCGAGGTTGGGTGAAAAAGGCGTACCGGTATCCCTGGATGAC
CTATAA

Structures

Toxin

• AlphaFold DB: F8G1E7

Antitoxin

• AlphaFold DB: F8G1E6